CD44 Antibody (9W6E7)

Name: CD44 Antibody (9W6E7)
Brand: Novus Biologicals
SKU: NBP3-15617
Price: 429 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-15617

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9W6E7 expressed in HEK293

View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-178 of human CD44 (NP_000601.3). AQIDLNITCRFAGVFHVEKNGRYSISRTEAADLCKAFNSTLPTMAQMEKALSIGFETCRYGFIEGHVVIPRIHPNSICAANNTGVYILTSNTSQYDTYCFNASAPPEEDCTSVTDLPNAFDGPITITIVNRDGTRYVQKGEYRTNPEDIYPSNPTDDDV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CD44 Antibody (9W6E7)

Western Blot: CD44 Antibody (9W6E7) [CD44] -

Western Blot: CD44 Antibody (9W6E7) [CD44] - Western blot analysis of various lysates using CD44 Rabbit mAb at 1:20000 dilution incubated overnight at 4C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.

Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] -

Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] -

Immunohistochemistry: CD44 Antibody (9W6E7) [CD44] - Immunohistochemistry analysis of paraffin-embedded Human skin using CD44 Rabbit mAb at dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (9W6E7) [CD44] -

Immunocytochemistry/ Immunofluorescence: CD44 Antibody (9W6E7) [CD44] - Confocal imaging of HeLa cells using CD44 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow Cytometry: CD44 Antibody (9W6E7) [CD44] -

Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 Jurkat cells (negative control,left) and HeLa cells were surface-stained with CD44 Rabbit mAb or ABflo 647 Rabbit IgG isotype control, followed by Alexa Fluor 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control.

Flow Cytometry: CD44 Antibody (9W6E7) [CD44] -

Flow Cytometry: CD44 Antibody (9W6E7) [CD44] - Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo 647 Rabbit IgG isotype control or CD44 Rabbit mAb.

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Applications for CD44 Antibody (9W6E7)

Application

Recommended Usage

Flow Cytometry

0.2ug per 10^6 cells in 100 ul

Immunocytochemistry/ Immunofluorescence

1:100 - 1:500

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Western Blot

1:1000 - 1:5000

Flow Cytometry Panel Builder

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (9W6E7)

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Certificate of Analysis

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Product Specific Notices for CD44 Antibody (9W6E7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.