CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free

Name: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-54571
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-54571

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # CD44v9/1459

Format

Azide and BSA Free

View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to the v9 domain of human CD44 (Uniprot: P16070)

Localization

Cell surface

Marker

Marker of Tumor Metastasis

Specificity

This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53204).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free [NBP2-54571]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) [NBP2-54571] - Formalin-paraffin human Tongue Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

Applications for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

Additional CD44 Products

Product Documents for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free

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Product Specific Notices for CD44 Antibody (CD44v9/1459) - variant 9 - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.