CD44 Antibody (CD44v9/1459) - variant 9

Novus Biologicals | Catalog # NBP2-53204

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # CD44v9/1459
View all CD44 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment corresponding to the v9 domain of human CD44 (Uniprot: P16070)

Localization

Cell surface

Marker

Marker of Tumor Metastasis

Specificity

This antibody recognizes an epitope encoded by exon v9 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54571)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for CD44 Antibody (CD44v9/1459) - variant 9

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-fixed, paraffin-embedded human Tongue Carcinoma stained with CD44 Antibody (CD44v9/1459) - variant 9.
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Cervical Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)
Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204]

Immunohistochemistry-Paraffin: CD44 Antibody (CD44v9/1459) - variant 9 [NBP2-53204] - Formalin-paraffin human Prostate Carcinoma stained with CD44 Monoclonal Antibody (CD44v9/1459)

Applications for CD44 Antibody (CD44v9/1459) - variant 9

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Use in WB reported in scientific literature (PMID:34490820)
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Reviewed Applications

Read 1 review rated 4 using NBP2-53204 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: CD44

CD44 represents a family of type I transmembrane glycoproteins expressed on many cell types such as B cells, monocytes, macrophages, and variable subsets of thymocytes and peripheral T cells. CD44 primarily functions as an adhesion molecule. It has been implicated in various processes, such as hematopoiesis, lymphocyte homing, leukocyte activation, tumor metastasis and development. The KM201 monoclonal antibody reacts with an epitope very close to the hyaluronate binding domain on the CD44 molecule. KM201 can inhibit hyaluronate-dependent cell aggregation, prevent lympho-hemopoiesis in both Dexter and Whitlock-Witte Cultures, prevent the earliest intrathymic precursors from homing to the thymus, and costimulate the activation of freshly purified splenic CD4+ T cells.1-6

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Gene Symbol

CD44

UniProt

P16070

Additional CD44 Products

Product Documents for CD44 Antibody (CD44v9/1459) - variant 9

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD44 Antibody (CD44v9/1459) - variant 9

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD44 Antibody (CD44v9/1459) - variant 9

Customer Reviews for CD44 Antibody (CD44v9/1459) - variant 9 (1)

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  • CD44 Antibody (CD44v9/1459) - variant 9
    Name: Xiuxia Zhou
    Application: Western Blot
    Sample Tested: airway epithelial cells
    Species: Human
    Verified Customer | Posted 04/29/2019
    1,3,5,7: unstimualted cells 2,4,6,8: IL-13 treated cells
    used 1:500 dilution for Western blot
    CD44 Antibody (CD44v9/1459) - variant 9 NBP2-53204

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