CD48/SLAMF2 Antibody (MEM-102) - BSA Free

Novus Biologicals | Catalog # NB500-391

Clone MEM-102 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # MEM-102

Format

BSA Free
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Product Specifications

Immunogen

Tissue / cell preparation (Human). (Burkitt's lymphoma cell line Raji).

Reactivity Notes

Cross-reacts with Human and Non Human Primates. Not yet tested in other species.

Specificity

CD48 antigen (human).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CD48/SLAMF2 Antibody (MEM-102) - BSA Free

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) [NB500-391] - Surface staining of human peripheral blood with anti-CD48 (MEM-102) PE.
Flow Cytometry: CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow Cytometry: CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow Cytometry: CD48/SLAMF2 Antibody (MEM-102) [NB500-391] - CD48 Antibody (MEM-102) [NB500-391]- Surface staining of human peripheral blood cells with anti-CD48 (MEM-102) Alexa Fluor 700.
Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) [NB500-391] - Surface staining of human peripheral blood with anti-CD48 (MEM-102) biotin / streptavidin-APC.
Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow (Cell Surface): CD48/SLAMF2 Antibody (MEM-102) [NB500-391] - Surface staining of human peripheral blood with anti-CD48 (MEM-102) Pacific BlueTM.
CD48/SLAMF2 Antibody (MEM-102) - BSA Free Flow Cytometry: CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Flow Cytometry: CD48/SLAMF2 Antibody (MEM-102) - BSA Free [NB500-391]

Separation of CD48 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in Analysis (surface staining) of human peripheral whole blood using anti-human CD48 (MEM-102) purified antibody (concentration in sample 3 ug/ml, GAM APC).

Applications for CD48/SLAMF2 Antibody (MEM-102) - BSA Free

Application
Recommended Usage

Flow Cytometry

2 ug/ml

Immunohistochemistry

5-10 ug/ml

Immunohistochemistry-Paraffin

1:200

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
FACS: Use at an assay dependant concentration. IHC-P: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Phosphate buffered saline (PBS), pH 7.4

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD48/SLAMF2

CD48 is a glycosyl phophatidyl inositol (GPI) anchored cell surface protein also known as Blast1, HuLy m3, BCM1 (in mouse), and OX45 (in rat). CD48 is strongly expressed on lymphocytes and monocytes and weakly on granulocytes but is absent on platelets, fibrolasts, epithelium and endothelium. CD48 is one of the markers for detecting the defects of GPI anchoring structure on patients with paroxysmal nocturnal hemoglobulinuria (PNH) and serves as a low affinity ligand for CD2. The CD48 antigen is closely related to the activation antigen, Blast 1.

Long Name

SLAM Family Member 2

Alternate Names

BCM1, BLAST1, CD48, MEM-102, SLAMF2

Gene Symbol

CD48

Additional CD48/SLAMF2 Products

Product Documents for CD48/SLAMF2 Antibody (MEM-102) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD48/SLAMF2 Antibody (MEM-102) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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