CD5 Antibody (H65) - BSA Free

Novus Biologicals | Catalog # NB100-2667

Clone H65 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Radioimmunoassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # H65

Format

BSA Free
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

HSB-2 cells.

Localization

Type I membrane protein

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for CD5 Antibody (H65) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Radioimmunoassay

1:100 - 1:2000

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD5

In humans, CD5 is a 67kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Entrez Gene IDs

921 (Human)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (H65) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (H65) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD5 Antibody (H65) - BSA Free

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Protocols

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FAQs

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