CD5 Antibody (OTI7A7)

Novus Biologicals | Catalog # NBP2-02690

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7A7
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD5 Antibody (OTI7A7)

Western Blot: CD5 Antibody (OTI7A7) [NBP2-02690]

Western Blot: CD5 Antibody (OTI7A7) [NBP2-02690]

Western Blot: CD5 Antibody (OTI7A7) [NBP2-02690] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunocytochemistry/Immunofluorescence: CD5 Antibody (OTI7A7) [NBP2-02690] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5.
Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690] - Staining of paraffin-embedded Human Kidney tissue using anti-CD5 mouse monoclonal antibody.
Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690]

Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690]

Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD5 antibody, and then analyzed by flow cytometry.
Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690]

Immunohistochemistry-Paraffin: CD5 Antibody (OTI7A7) [NBP2-02690] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD5 mouse monoclonal antibody.
Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690]

Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690]

Flow Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690] - Analysis of Jurkat cells, using anti-CD5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
CD5 Antibody (OTI7A7)

Imaging Mass Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690] -

Imaging Mass Cytometry: CD5 Antibody (OTI7A7) [NBP2-02690] - Human tonsil tissue, CD5 conjugated to Er167. IMC image submitted by a verified customer review.

Applications for CD5 Antibody (OTI7A7)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.68 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Entrez Gene IDs

921 (Human)

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (OTI7A7)

Certificate of Analysis

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Product Specific Notices for CD5 Antibody (OTI7A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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