CD5 Antibody (OTI7A7) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-70360

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Imaging Mass Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7A7

Format

Azide and BSA Free
View all CD5 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CD5 (NP_055022) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

54.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CD5 Antibody (OTI7A7) - Azide and BSA Free (NBP2-02690) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CD5 Antibody (OTI7A7) - Azide and BSA Free

Western Blot: CD5 Antibody (OTI7A7)Azide and BSA Free [NBP2-70360]

Western Blot: CD5 Antibody (OTI7A7)Azide and BSA Free [NBP2-70360]

Western Blot: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD5.
Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunocytochemistry/ Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunocytochemistry/Immunofluorescence: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CD5.
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Human Kidney tissue using anti-CD5 mouse monoclonal antibody.
Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-CD5 antibody, and then analyzed by flow cytometry.
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-CD5 mouse monoclonal antibody.
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-CD5 mouse monoclonal antibody.
Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Immunohistochemistry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-CD5 mouse monoclonal antibody.
Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Flow Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Analysis of Jurkat cells, using anti-CD5 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360]

Imaging Mass Cytometry: CD5 Antibody (OTI7A7) - Azide and BSA Free [NBP2-70360] - Human tonsil tissue, CD5 conjugated to Er167. IMC image submitted by a verified customer review.

Applications for CD5 Antibody (OTI7A7) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Imaging Mass Cytometry

Validated from a verified customer review

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Reviewed Applications

Read 1 review rated 4 using NBP2-70360 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD5

In humans, CD5 is a 55kDa T lymphocyte single chain transmembrane glycoprotein. It is present on all mature T lymphocytes, on most thymocytes and on many T cell leukemias and lymphomas. It reacts with a subpopulation of activated B cells. CD5/Lyt1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells. It has been identified as the major ligand of the B cell antigen CD72. The frequency of CD5+ B cells exhibits strain dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B1 cells. Binding of CD5 on the T cell surface can augment alloantigen or mitogen induced lymphocyte proliferation and induces increased cytosolic free calcium, IL2 secretion, and IL2R expression. It has been proposed that CD5 negatively regulates signal transduction mediated by the T cell and B cell receptors.

Alternate Names

CD5

Gene Symbol

CD5

Additional CD5 Products

Product Documents for CD5 Antibody (OTI7A7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CD5 Antibody (OTI7A7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for CD5 Antibody (OTI7A7) - Azide and BSA Free (1)

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  • CD5 Antibody (OTI7A7) - Azide and BSA Free
    Name: Anonymous
    Application: Imaging Mass Cytometry
    Sample Tested: Human Tonsil tissue
    Species: Human
    Verified Customer | Posted 04/09/2021
    IMC image, human tonsil tissue, CD5 conjugated to Er167
    CD5 Antibody (OTI7A7) - Azide and BSA Free NBP2-70360

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Protocols

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FAQs

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