CD69 Antibody (8B6)

Novus Biologicals | Catalog # NBP1-51607

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Imaging Mass Cytometry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8B6
View all CD69 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human CD69 expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

22.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CD69 Antibody (8B6)

Western Blot: CD69 Antibody (8B6)BSA Free [NBP1-51607]

Western Blot: CD69 Antibody (8B6)BSA Free [NBP1-51607]

Western Blot: CD69 Antibody (8B6) [NBP1-51607] - Analysis using CD69 mouse mAb against, Jurkat (1), L1210 (2) and TPH-1 (3) cell lysate.
Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) [NBP1-51607] - Human tonsil tissue stained with CD69 antibody NBP1-51607. IHC-P image submitted by a verified customer review.
Flow Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Flow Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Flow Cytometry: CD69 Antibody (8B6) [NBP1-51607] - Analysis of Jurkat cells using CD69 mouse mAb (green) and negative control (purple).
Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Immunohistochemistry-Paraffin: CD69 Antibody (8B6) [NBP1-51607] - Analysis of human Tonsil tissues using anti-CD69 mouse mAb
Imaging Mass Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Imaging Mass Cytometry: CD69 Antibody (8B6) - BSA Free [NBP1-51607]

Imaging Mass Cytometry: CD69 Antibody (8B6) [NBP1-51607] - Human bone marrow FFPE tissue section. DNA stained in blue, CD69 stained in white. IMC image submitted by a verified customer review.

Applications for CD69 Antibody (8B6)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Application Notes
CD69 antibody validated for IHC-P and Imaging Mass Cytometry from verified customer reviews.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-51607 in the following applications:

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD69

CD69 is a 60 kD type II membrane protein composed of a 27/33 kD disulfide-linked homodimer, also known as Very Early Activation Antigen (VEA), AIM, EA1, MLR3 and gp34/28. It is expressed on a subset of thymocytes and platelets. CD69 is rapidly induced on activated T and B cells, neutrophils, and NK cells. It is a C-type lectin, closely related to the NKR-P1 and Ly-49 NK cell activation molecules. CD69 is involved in the early events of cell activation and thymocyte positive selection. The H1.2F3 antibody has been reported to augment T cell activation.

Alternate Names

CD69, EA-1, Leu23, MLR-3, p60

Gene Symbol

CD69

Additional CD69 Products

Product Documents for CD69 Antibody (8B6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CD69 Antibody (8B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD69 Antibody (8B6)

Customer Reviews for CD69 Antibody (8B6) (2)

4 out of 5
2 Customer Ratings
5 Stars
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4 Stars
100%
3 Stars
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1 Stars
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Customer Images

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  • CD69 Antibody (8B6)
    Name: Michela Colombo
    Application: Imaging Mass Cytometry
    Sample Tested: bone marrow
    Species: Human
    Verified Customer | Posted 11/12/2021
    IMC on human bone marrow FFPE slides. DNA-blue CD69-white
    CD69 Antibody (8B6) NBP1-51607
    Bio-Techne Response
    This review was submitted through the legacy Novus Innovators Program, reflecting a new species or application tested on a primary antibody.
  • CD69 Antibody (8B6)
    Name: Masahiro Hirata
    Application: Immunohistochemistry-Paraffin
    Sample Tested: human tonsil
    Species: Human
    Verified Customer | Posted 09/11/2020
    8B6 was reacted to activated lymphocytes
    Pretreatment: HIER, ER2(Leica) Primary Ab: 1:1000 Detection system: BOND polymer refine detection kit (Leica)
    CD69 Antibody (8B6) NBP1-51607

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Protocols

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FAQs

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