CD81 Antibody (1D6) - BSA Free

Novus Biologicals | Catalog # NB100-65805

Clone 1D6 was used by HLDA to establish CD designation.
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Goat, Primate, Sheep

Cited:

Human, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Dot Blot, CyTOF-ready

Cited:

Western Blot, ELISA, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Cytometric Bead Assay Standard

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D6

Format

BSA Free
View all CD81 Primary Antibodies »

Product Specifications

Immunogen

Aggregated OCI-Ly8 human B cell line

Reactivity Notes

Mouse reactivity reported in scientific literature (Ji et al). Canine reactivity reported from a verified customer review. Primate reactivity reported in scientific literature (PMID:33077711).

Localization

Membrane; Multi-pass membrane protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CD81 Antibody (1D6) - BSA Free

Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

CD81-Antibody-1D6-Western-Blot-NB100-65805-img0015.jpg
Immunohistochemistry-Paraffin: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Immunohistochemistry-Paraffin: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Immunohistochemistry-Paraffin: CD81 Antibody (1D6) [NB100-65805] - Staining of TAPA1 in human testis using DAB with hematoxylin counterstain.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody (1D6) NB100-65805 (blue) and a matched isotype control MAB002 (orange). Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).
Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

Western Blot: CD81 Antibody (1D6)BSA Free [NB100-65805]

Western Blot: CD81 Antibody (1D6) [NB100-65805] - Analysis of human testis tissue (A) using CD81 antibody at a concentration of 2 ug/mL.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Surface flow cytometric staining of 1 x 10^6 PBMC cells using CD81 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1 x 10^6 cells was used.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - Analysis using the FITC conjugate of NB100-65805. Staining of human peripheral blood lymphocytes.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 (1D6) antibody NB100-65805AF647(blue) and a matched isotype control NBP2-27287AF647 (orange). Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat Cells with CD81 Antibody (1D6) NB100-65805B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 1 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) - BSA Free [NB100-65805]

Flow Cytometry: CD81 Antibody (1D6) [NB100-65805] - A surface stain was performed on Jurkat cells with CD81 Antibody [1D6] NB100-65805AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
CD81 Antibody (1D6) - BSA Free

Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] -

Western Blot: CD81 Antibody (1D6) - BSA Free [NB100-65805] - Characterization & internalization of M2-Exos. (A) TEM image of M2-Exos. Scale bar: 200 nm. (B) DLS measurement of M2-Exos size. (C) Western blotting assay of exosomal markers in THP-1-M2 cellular lysate & M2-Exos preparation. (D,F) Fluorescence images of 786-O & ACHN cells treated with or without PKH67-labeled M2-Exos (green). Scale bar: 50 μm. (E,G) Three-dimensional confocal reconstruction of 786-O & ACHN cells treated with PKH67-labeled M2-Exos (green). (H) Fluorescence staining analyzing the internalization of M2-Exos by 786-O cells over 12 h. Scale bar: 10 μm. CT: control. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35328425), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CD81 Antibody (1D6) - BSA Free

Immunocytochemistry/ Immunofluorescence: CD81 Antibody (1D6) - BSA Free [NB100-65805] -

Principle of EV‐GLYPH assay for early‐stage NSCLC identification. A) Glycan signatures on sEVs derived from patients with benign and malignant lung diseases. B) CT and PET images of benign and malignant lung nodules. C) Working scheme of EV‐GLYPH assay. SEC‐purified sEVs from plasma are captured by anti‐MUC1 antibody immobilized on an electrode and subsequently labeled with SERS nanotags against LacdiNAc, T antigen, and CD81. D) A nanomixing fluid flow is generated on the electrode surface by the applied ac‐EHD field. E) With in situ SERS mapping, the average Raman intensities and false‐color SERS spectral images (insets) are established based on the characteristic Raman signals of three SERS nanotags (WFA‐MBA, 1075 cm−1, red; PNA‐TFMBA, 1375 cm−1, blue; anti‐CD81‐DTNB, 1335 cm−1, green), representing the expression of LacdiNAc, T antigen, and CD81, respectively. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38885350), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CD81 Antibody (1D6) - BSA Free

Application
Recommended Usage

Flow Cytometry

0.2 - 1 ug per million cells

Immunocytochemistry/ Immunofluorescence

1:10 - 1:100

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10 - 1:500

Western Blot

1 - 2 ug/mL

Reviewed Applications

Read 1 review rated 5 using NB100-65805 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CD81

TAPA1, also known as CD81, is found in the plasma membrane in lymphocytes and is believed to play an important role in the regulation of lymphoma cell growth. This protein is primarily found on B and T cells (lymphocytes) in humans. TAPA1 appears to be involved in a variety of cellular processes including activation, proliferation and differentiation. It is believed to act as a viral receptor for HCV and may play a crucial role in cell signaling from its place embedded in the plasma membrane. Mutations affecting CD81 can cause immunodeficiency- a disease that is characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections due to a lack of an immune response.

Alternate Names

CD81, TAPA-1, TSPAN28

Gene Symbol

CD81

Additional CD81 Products

Product Documents for CD81 Antibody (1D6) - BSA Free

Certificate of Analysis

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Product Specific Notices for CD81 Antibody (1D6) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CD81 Antibody (1D6) - BSA Free

Customer Reviews for CD81 Antibody (1D6) - BSA Free (1)

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Customer Images

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  • CD81 Antibody (1D6)
    Name: Anonymous
    Application: Dot Blot
    Sample Tested: EVs from canine umbilical cord-derived mesenchymal stromal cells, Cell lysate from canine umbilical cord-derived mesenchymal stromal cells, cell lysate canine umbilical cord-derived mesenchymal stromal cells and EVs derived from canine umbilical cord-derived mesenchymal stromal cells
    Species: Canine
    Verified Customer | Posted 10/09/2020
    Dot Blot of EVs derived from canine umbilical cord-derived mesenchymal stromal cells (6 dots, starting at top). Positive control: total cell lysate from canine umbilical cord-derived mesenchymal stromal cells.Negative control: water.
    CD81 Antibody (1D6) - BSA Free NB100-65805

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