CD81 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] -

Immunocytochemistry/ Immunofluorescence: CD81 Antibody - BSA Free [NBP1-77039] - Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 ug/mL.

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] -

Immunohistochemistry: CD81 Antibody - BSA Free [NBP1-77039] - Immunohistochemistry of CD81 in human brain tissue with CD81 antibody at 2 ug/ml.

Western Blot: CD81 Antibody - BSA Free [NBP1-77039] -

Blood plasma sEV from high fertile and low-fertile both confirmed sEV characteristics and differ in total protein concentrations. (A) Total protein content of sEV from low fertile dairy cow plasma are significantly higher than the high fertile. Values are presented as mean +/- SD (**P < 0.001; Mann–Whitney test). (B) However, there’s no significant difference between the particle number (yield) according to the nano-particle tracking analysis (NTA) results. Values are presented as mean +/- SD (**P < 0.001; Mann–Whitney test). (C) Representation of western blot sEV/exosome marker CD81, FLOT-1 and negative sEV/exosome marker BSA for pooled sEV fractions 7–10 of HF-EXO and LF-EX. Presence of FLOT-1 and CD81 and less abundance of BSA confirmed sEV/exosome. The full western blot images are available in the Supplementary File 3 (D) The size of sEV (nm) is within the defined size (30–200 nm)—average nano-particle tracking analysis (NTA) particle size distribution of HF-EXO and LF-Exo biological replicates (n = 10 each). There was no statistically significant difference between HF-EXO and LF-EXO particle size distributions (ns represents P > 0.05; Mann–Whitney test). (E) Spherical shape was confirmed in electron micrographs of exosomes from both (i) HF-EXO and (ii) LF-EXO. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37012302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CD81 Antibody - BSA Free [NBP1-77039] -

Hebp1 is delivered by MCP-EVs and contributes to nerve regeneration. (A) Representative transmission electron microscopy (TEM) phase images for detecting isolated MCP-EVs. Scale bar = 100 nm. (B) Representative Western blot for Hebp1, EV positive markers (CD63, CD9 and CD81) or EV negative marker (GM130) in MCPs and MCP-EVs. (C, D) Expression of Hebp1 in MCP-EVs infected with lentivirus expressing shHebp1 or shCon at three doses (1 × 103, 5 × 104, and 1 × 104 TU/ml culture medium) for at least 3 days. (E, F) Densitometric quantification of Hebp1 protein bands using an image analyzer. Results are presented as means +/- SEM (n = 4). (G, H) Immunofluorescence staining for NF in mouse MPG tissues exposed to LPS (2.5 μg/ml) and other indicated conditions for 5 days (G). Lengths of NF-positive neurites in MPG tissues, quantified using an image analyzer (H). Results are presented as means +/- SEM (n = 4). Scale bar, 100 um. (I, J) Migration assays of mouse Schwann cells exposed to LPS (2.5 μg/ml) and other indicated conditions for 24 hours (I). Number of migrated cells in the red dotted rectangle, quantified using an image analyzer (J). Results are presented as means +/- SEM (n = 4; **P < 0.01, ***P < 0.001). The relative ratio of the shCon (control) group was defined as 1. MCPs, mouse cavernous pericytes; EVs, extracellular vesicles; LPS, lipopolysaccharide; MPG, major pelvic ganglion; PBS, phosphate-buffered saline; ns, not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37324943), licensed under a CC-BY license. Not internally tested by Novus Biologicals.