CDC2/CDK1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85729

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CDC2/CDK1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LGSARYSTPVDIWSIGTIFAELATKKPLFHGDSEIDQLFRIFRALGTPNNEVWPEVESLQDYKNTFPKWKPGSLASHVKNLDENGLDLLSKMLIYDPAKRISGKMALNHPYFNDLDNQIKKM

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CDC2/CDK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining in human tonsil and pancreas tissues. Corresponding CDK1 RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729]

Immunocytochemistry/ Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729]

Immunocytochemistry/Immunofluorescence: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human cell line U-251 MG shows localization to nucleus & cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human tonsil shows moderate cytoplasmic positivity in germinal center cells.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human skin shows moderate cytoplasmic positivity in epidermal cells.
Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729]

Immunohistochemistry-Paraffin: CDC2/CDK1 Antibody [NBP1-85729] - Staining of human pancreas shows no cytoplasmic positivity in exocrine glandular cells as expected.
CDC2/CDK1 Antibody - BSA Free Western Blot: CDC2/CDK1 Antibody - BSA Free [NBP1-85729]

Western Blot: CDC2/CDK1 Antibody - BSA Free [NBP1-85729]

Analysis in human cell line A-431.
CDC2/CDK1 Antibody - BSA Free Western Blot: CDC2/CDK1 Antibody - BSA Free [NBP1-85729]

Western Blot: CDC2/CDK1 Antibody - BSA Free [NBP1-85729]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for CDC2/CDK1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDC2/CDK1

The cell division control protein cdc2, also known as cyclin-dependent kinase 1 (Cdk1) or p34/cdk1, plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the cdc25 phosphatase. The isoform CDC2deltaT is found in breast cancer tissues. Furthermore, cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration.

Long Name

Cell Division Cycle 2/Cyclin-dependent Kinase 1

Alternate Names

CDK1

Gene Symbol

CDK1

Additional CDC2/CDK1 Products

Product Documents for CDC2/CDK1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDC2/CDK1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

Citations for CDC2/CDK1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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