CDK2 Antibody (CL7990) - BSA Free

Novus Biologicals | Catalog # NBP2-88911

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL7990

Format

BSA Free
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from P24941, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CDK2 Antibody (CL7990) - BSA Free

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Analysis in human placenta and kidney tissues using NBP2-88911 antibody. Corresponding CDK2 RNA-seq data are presented for the same tissues.
Western Blot: CDK2 Antibody (CL7990) [NBP2-88911]

Western Blot: CDK2 Antibody (CL7990) [NBP2-88911]

Western Blot: CDK2 Antibody (CL7990) [NBP2-88911] - Analysis in human cell line SK-MEL-30.
Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (CL7990) [NBP2-88911]

Immunocytochemistry/ Immunofluorescence: CDK2 Antibody (CL7990) [NBP2-88911]

Immunocytochemistry/Immunofluorescence: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of SK-MEL-30 cells using the Anti-CDK2 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human kidney shows no positivity in cells in tubules as expected.
Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911]

Immunohistochemistry-Paraffin: CDK2 Antibody (CL7990) [NBP2-88911] - Staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.

Applications for CDK2 Antibody (CL7990) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 Antibody (CL7990) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDK2 Antibody (CL7990) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail