CDK2 Antibody (CL7990) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44198

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL7990

Format

Azide and BSA Free
View all CDK2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P24941, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CDK2 Antibody (CL7990) - Azide and BSA Free

CDK2 Antibody (CL7990) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
CDK2 Antibody (CL7990) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Analysis in human placenta and kidney tissues using NBP3-44198 antibody. Corresponding CDK2 RNA-seq data are presented for the same tissues.
CDK2 Antibody (CL7990) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Staining of human kidney shows no positivity in cells in tubules as expected.
CDK2 Antibody (CL7990) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Staining of human renal cancer shows moderate to strong nuclear positivity in tumor cells.
CDK2 Antibody (CL7990) - Azide and BSA Free Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Immunohistochemistry-Paraffin: CDK2 Antibody [NBP3-44198]

Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
CDK2 Antibody (CL7990) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: CDK2 Antibody [NBP3-44198]

Immunocytochemistry/ Immunofluorescence: CDK2 Antibody [NBP3-44198]

Staining of SK-MEL-30 cells using the Anti-CDK2 monoclonal antibody).
CDK2 Antibody (CL7990) - Azide and BSA Free Western Blot: CDK2 Antibody [NBP3-44198]

Western Blot: CDK2 Antibody [NBP3-44198]

Analysis in human cell line SK-MEL-30.

Applications for CDK2 Antibody (CL7990) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDK2

The protein encoded by the Cdk2 gene is a member of the Ser/Thr protein kinase family. This protein kinase is highlysimilar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of thecyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycleG1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex includingcyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its proteinphosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have beenreported. (provided by RefSeq)

Long Name

Cyclin-dependent Kinase 2

Alternate Names

cdc2-related protein kinase, cell devision kinase 2, Cell division protein kinase 2, cyclin-dependent kinase 2, EC 2.7.11, EC 2.7.11.22, p33 protein kinase, p33(CDK2)

Gene Symbol

CDK2

Additional CDK2 Products

Product Documents for CDK2 Antibody (CL7990) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CDK2 Antibody (CL7990) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cell Cycle
Intracellular Kinases

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Protocols

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FAQs

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Associated Pathways

Akt Signaling Pathway Akt Signaling Pathway Thumbnail