CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

Novus Biologicals | Catalog # H00001523-M01

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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2A10

Format

Azide and BSA Free
View all CDP/CUTL1 Primary Antibodies »

Product Specifications

Immunogen

CUTL1 (NP_001904.2, 521 a.a. ~ 620 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AENRLAQHTLQALQSELDSLRADNIKLFEKIKFLQSYPGRGSGSDDTELRYSSQYEERLDPFSSFSKRERQRKYLSLSPWDKATLSMGRLVLSNKMARTI

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

CUTL1 - cut-like 1, CCAAT displacement protein (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

Western Blot: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Western Blot: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Western Blot: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of CUX1 expression in transfected 293T cell line by CUTL1 monoclonal antibody (M01), clone 2A10.Lane 1: CUX1 transfected lysate(77.2 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunocytochemistry/ Immunofluorescence: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunocytochemistry/Immunofluorescence: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of monoclonal antibody to CUTL1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of monoclonal antibody to CUTL1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. Antibody concentration 5 ug/ml.
Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunohistochemistry-Paraffin: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - mouse cortex - postnatal brain was stained with anti-CUTL1 antibody. Image from verified customer review.
Immunoprecipitation: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunoprecipitation: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

Immunoprecipitation: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Analysis of CUX1 transfected lysate using anti-CUX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CUX1 MaxPab rabbit polyclonal antibody.
ELISA: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

ELISA: CDP/CUTL1 Antibody (2A10) [H00001523-M01]

ELISA: CDP/CUTL1 Antibody (2A10) [H00001523-M01] - Detection limit for recombinant GST tagged CUTL1 is approximately 0.03ng/ml as a capture antibody.

Applications for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB.

Reviewed Applications

Read 1 review rated 4 using H00001523-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDP/CUTL1

The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

CCAAT Displacement protein/CUT-like Homeobox Protein 1

Alternate Names

CASP, COY1, CUTL1, CUX, CUX1, GOLIM6

Entrez Gene IDs

1523 (Human)

Gene Symbol

CUX1

OMIM

116896 (Human)

UniProt

AAH25422 (Human)

Additional CDP/CUTL1 Products

Product Documents for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

Customer Reviews for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free (1)

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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: mouse brain
    Species: Mouse
    Verified Customer | Posted 10/03/2014
    Cortex - postnatal brain mouse
    CDP/CUTL1 Antibody (2A10) - Azide and BSA Free H00001523-M01

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Protocols

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FAQs for CDP/CUTL1 Antibody (2A10) - Azide and BSA Free

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  • Q: We are intending to do a ChIP with CUX1 (p75 isoform) and would therefore be interested in your antibody (Cat. # H00001523-M01). Has it been shown that it recognizes the p75 isoform of CUX1? (http://www.ncbi.nlm.nih.gov/protein/NP_852477.1) Are there any references or data you could send us that can confirm that this antibody would be suitable for a ChIP? Or do you have a different antibody that works for our desired application?

    A: This antibody is produced by a Taiwanese company called Abnova and we distribute it for them. We do no in-house testing or development of their products. The immunogen for this antibody is present in the p75 isoform of CUX1, so it will recognize this isoform. It has successfully been used in IP, so it should work just fine in ChIP. The use of the antibody in these two applications is essentially the same, so there should be no problems.

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