CDS1 Antibody (2D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00001040-M01

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Rat

Cited:

Human

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2D10

Format

Azide and BSA Free
View all CDS1 Primary Antibodies »

Product Specifications

Immunogen

CDS1 (NP_001254, 1 a.a. ~ 98 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLELRHRGSCPGPREAVSPPHREGEAAGGDHETESTSDKETDIDDRYGDLDSRTDSDIPEIPPSSDRTPEILKKALSGLSSRWKNWWIRGILTLTMIS

Reactivity Notes

Rat reactivity reported from customer review.

Specificity

CDS1 - CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1 (2D10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for CDS1 Antibody (2D10) - Azide and BSA Free

Western Blot: CDS1 Antibody (2D10) [H00001040-M01]

Western Blot: CDS1 Antibody (2D10) [H00001040-M01]

CDS1-Antibody-2D10-Western-Blot-H00001040-M01-img0009.jpg
Immunocytochemistry/ Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01]

Immunocytochemistry/ Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01]

Immunocytochemistry/Immunofluorescence: CDS1 Antibody (2D10) [H00001040-M01] - Analysis of monoclonal antibody to CDS1 on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: CDS1 Antibody (2D10) [H00001040-M01]

Western Blot: CDS1 Antibody (2D10) [H00001040-M01]

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - CDS1 monoclonal antibody (M01), clone 2D10. Analysis of CDS1 expression in human liver.
ELISA: CDS1 Antibody (2D10) [H00001040-M01]

ELISA: CDS1 Antibody (2D10) [H00001040-M01]

ELISA: CDS1 Antibody (2D10) [H00001040-M01] - Detection limit for recombinant GST tagged CDS1 is 0.1 ng/ml as a capture antibody.
Knockdown Validated: CDS1 Antibody (2D10) [H00001040-M01]

Western Blot: CDS1 Antibody (2D10) [H00001040-M01]

CDS1-Antibody-2D10-Knockdown-Validated-H00001040-M01-img0010.jpg
CDS1 Antibody (2D10)

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] -

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CDS1 Antibody (2D10)

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] -

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CDS1 Antibody (2D10)

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] -

Western Blot: CDS1 Antibody (2D10) [H00001040-M01] - Localisation of CDS1 immunoreactivity & CDS activity to mitochondria.[A, B] Differentiated H9c2 cells fractionated & analysed for [A] CDS1 immunoreactivity by Western blot; [B] CDS activity. [C-E] Rat heart fractionated & analysed for [C] CDS1 immunoreactivity & markers, [D] CDS1 immunoreactivity (entire Western blot shown) & [E] CDS activity. [F–H] Rat heart fractionated in the presence of the protease, subtilisin & analysed for [F] CDS1 immunoreactivity by western blot, [G] CDS activity & [H] PI synthase (PIS) activity. CDS & PIS activity was monitored in triplicate & error bars denote ± S.E.M. COXIV, GRP75 & cyto c are markers for mitochondria, PITPNC1 & PITP alpha are cytosolic markers & calnexin is a marker for the ER. WCL, whole cell lysate; Micro, microsomes; C.Mito, crude mitochondria; Cyto, cytosol; P.Mito, pure mitochondria, MAMs, mitochondrial associated membranes; PIS, PI synthase; CDS, CDP-diacylglycerol synthase. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29253589), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CDS1 Antibody (2D10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Reviewed Applications

Read 1 review rated 4 using H00001040-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CDS1

Breakdown products of phosphoinositides are ubiquitous second messengers that function downstream of many G protein-coupled receptors and tyrosine kinases regulating cell growth, calcium metabolism, and protein kinase C activity. This gene encodes an enzyme which regulates the amount of phosphatidylinositol available for signaling by catalyzing the conversion of phosphatidic acid to CDP-diacylglycerol. This enzyme is an integral membrane protein localized to two subcellular domains, the matrix side of the inner mitochondrial membrane where it is thought to be involved in the synthesis of phosphatidylglycerol and cardiolipin and the cytoplasmic side of the endoplasmic reticulum where it functions in phosphatidylinositol biosynthesis. Two genes encoding this enzyme have been identified in humans, one mapping to human chromosome 4q21 and a second to 20p13. [provided by RefSeq]

Alternate Names

CDP-DAG synthase 1, CDP-DG synthase 1, CDP-DG synthetase 1, CDP-diacylglycerol synthase (phosphatidate cytidylyltransferase) 1, CDP-diacylglycerol synthase 1, CDP-diglyceride pyrophosphorylase 1, CDP-diglyceride synthase 1, CDP-diglyceride synthetase 1, CDS, CDS 1, CTP:phosphatidate cytidylyltransferase 1, EC 2.7.7.41, phosphatidate cytidylyltransferase 1

Entrez Gene IDs

1040 (Human)

Gene Symbol

CDS1

UniProt

NP_001254 (Human)

Additional CDS1 Products

Product Documents for CDS1 Antibody (2D10) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CDS1 Antibody (2D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CDS1 Antibody (2D10) - Azide and BSA Free

Customer Reviews for CDS1 Antibody (2D10) - Azide and BSA Free (1)

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Customer Images

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  • Name: Bryan Tinsley
    Application: Western Blot
    Sample Tested:
    Species: Rat
    Verified Customer | Posted 09/08/2014
    CDS1 antibody on various cell lines
    CDS1 Antibody (2D10) - Azide and BSA Free H00001040-M01

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Protocols

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