CEACAM8/CD66b Antibody (6/40c) - BSA Free

Novus Biologicals | Catalog # NBP2-54627

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 6/40c

Format

BSA Free
View all CEACAM-8/CD66b Primary Antibodies »

Product Specifications

Immunogen

Recombinant soluble human CEACAM8-Fc produced in HEK293 cells

Specificity

Clone 6/40c does not cross-react with human CEACAM1/3/4/5/6/7, rat CECAM1, or mouse CEACAM1/2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CEACAM8/CD66b Antibody (6/40c) - BSA Free

Western Blot: CEACAM8/CD66b Antibody (6/40c)BSA Free [NBP2-54627]

Western Blot: CEACAM8/CD66b Antibody (6/40c)BSA Free [NBP2-54627]

Western Blot: CEACAM8/CD66b Antibody (6/40c) [NBP2-54627] - Western blot analysis of human granulocyte lysate: Detection using 10 ug/ml Anti-CEACAM8 [6/40c] followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.
Flow Cytometry: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

Flow Cytometry: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

Flow Cytometry: CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Binding of 10 ug/ml primary positive control mAb (upper panel), Anti-CEACAM8 [6/40c] (lower panel), negative isotype matched control mAb (thin line) on different CHO-human CEACAM transfected cells (the CEACAM family member expressed is stated above).
ELISA: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

ELISA: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

ELISA: CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Solid phase was coated with 3 ug/ml Anti-CEA binding human CEACAM1-CEACAM8. After washing, blocking and coating human CEACAM1 antigen, detecting antibody Anti-CEACAM8 [6/40c] (10 ug/ml) followed by HRP-coupled goat anti-mouse Ig was added. TMB was used for visualizing the binding measured by Tecan-ELISA reader at 450 nm.
Immunoprecipitation: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

Immunoprecipitation: CEACAM8/CD66b Antibody (6/40c) - BSA Free [NBP2-54627]

Immunoprecipitation: CEACAM8/CD66b Antibody (6/40c) [NBP2-54627] - CEACAM-8/CD66b Antibody (6/40c) [NBP2-54627] - Analysis in human granulocyte lysate: Utilizing 10 ug/ml Anti-CEACAM8 [6/40c]. Detection of the sample using 5 ug/ml pAb anti CEA (Dako) followed by HRP-coupled secondary pAb. Visualized by chemiluminescence detection.

Applications for CEACAM8/CD66b Antibody (6/40c) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Flow Cytometry

1:10 - 1:1000

Immunohistochemistry

1:100 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -70C long term. Avoid freeze-thaw cycles.

Background: CEACAM-8/CD66b

CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67, CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily, carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been reported to induce activation in neutrophils and to be involved in heterophilic adhesion with CD66c.

Long Name

Carcinoembryonic Antigen-related Cell Adhesion Molecule 8

Alternate Names

CD66b, CD67, CEACAM8, CGM6, NCA-95, anti human cd66b apc, cd66b clone g10f5, cd66b G10F5, cd66b granulocyte marker, cd66b ihc, cd66b marker, cd66b neutrophil marker, cd66b neutrophils

Gene Symbol

CEACAM8

Additional CEACAM-8/CD66b Products

Product Documents for CEACAM8/CD66b Antibody (6/40c) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CEACAM8/CD66b Antibody (6/40c) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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