CEBP alpha Antibody (5B7)

Novus Biologicals | Catalog # NBP2-37497

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B7
View all CEBP alpha Primary Antibodies »

Product Specifications

Immunogen

Synthesized peptide of human CEBP alpha (AA: C-RKSRDKAKRNVETKV).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse CEBP alpha Antibody (5B7) (NBP2-37497) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CEBP alpha Antibody (5B7)

Western Blot: CEBP alpha Antibody (5B7)BSA Free [NBP2-37497]

Western Blot: CEBP alpha Antibody (5B7)BSA Free [NBP2-37497]

Western Blot: CEBP alpha Antibody (5B7) [NBP2-37497] - Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunohistochemistry: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunohistochemistry: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBPA mouse mAb with DAB staining.
Flow Cytometry: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Flow Cytometry: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Flow Cytometry: CEBP alpha Antibody (5B7) [NBP2-37497] - Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and negative control (red).
Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

Immunocytochemistry/Immunofluorescence: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

ELISA: CEBP alpha Antibody (5B7) - BSA Free [NBP2-37497]

ELISA: CEBP alpha Antibody (5B7) [NBP2-37497] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Applications for CEBP alpha Antibody (5B7)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CEBP alpha

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Long Name

CCAAT/enhancer-Binding Protein alpha

Alternate Names

C/EBP-alpha, CEBPA

Gene Symbol

CEBPA

Additional CEBP alpha Products

Product Documents for CEBP alpha Antibody (5B7)

Certificate of Analysis

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Product Specific Notices for CEBP alpha Antibody (5B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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