Cell Cycle Regulation Antibody Pack

Detection of CDK2 in HEK293, HeLa, K562 and Mouse Embryonic Fibroblast lysates by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse CDK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4654) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). For additional reference, recombinant human CDK2, CDK4, and CDK6 (5 ng/lane) were included. A specific band for CDK2 was detected at approximately 34 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of p27/Kip1 in NRK, U2O2 and Mouse Embryonic Fibroblast lysates by Western Blot.

Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat p27/Kip1 Monoclonal Antibody (Catalog # MAB2256) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Cyclin D1 in C2C12 and NIH3T3 lysates by Western Blot.

Western blot shows lysates of 1) mouse C2C12 and 2) mouse NIH 3T3 lysate. PVDF membrane was probed with 5 µg/mL of Rabbit Anti-Human Cyclin D1 Polyclonal Antibody (Catalog # NBP2-24695) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody. This experiment was conducted under reducing conditions. PicoTect ECL substrate solution were used for this test.