Centrin 2 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-92653

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Knockout Validated, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all Centrin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-172 of human CETN2 (NP_004335.1). MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

20 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Centrin 2 Antibody - Azide and BSA Free

Western Blot: Centrin 2 AntibodyAzide and BSA Free [NBP2-92653]

Western Blot: Centrin 2 AntibodyAzide and BSA Free [NBP2-92653]

Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts of various cell lines, using CETN2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.
Knockout Validated: Centrin 2 Antibody - Azide and BSA Free [NBP2-92653]

Western Blot: Centrin 2 Antibody - Azide and BSA Free [NBP2-92653]

Western Blot: Centrin 2 Antibody [NBP2-92653] - Analysis of extracts from normal (control) and CETN2 knockout (KO) HeLa cells, using CETN2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Applications for Centrin 2 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Centrin 2

Alternate Names

Caltractin isoform 1, CEN2CALTcaltractin (20kD calcium-binding protein), centrin, EF-hand protein, 2, centrin-2

Gene Symbol

CETN2

Additional Centrin 2 Products

Product Documents for Centrin 2 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Centrin 2 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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