CGI-16 Antibody (4E4) - Azide and BSA Free

Novus Biologicals | Catalog # H00023597-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4E4

Format

Azide and BSA Free
View all CGI-16 Primary Antibodies »

Product Specifications

Immunogen

ACATE2 (AAH12573, 1 a.a. ~ 212 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRRAALRLCALGKGQLTPGRGLTQGPQNPKKQGIFHIHEVRDKLREIVGASTNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKG

Reactivity Notes

Human. Other species not tested.

Specificity

ACOT9 - acyl-Coenzyme A thioesterase 2, mitochondrial

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for CGI-16 Antibody (4E4) - Azide and BSA Free

Western Blot: CGI-16 Antibody (4E4) [H00023597-M01]

Western Blot: CGI-16 Antibody (4E4) [H00023597-M01]

Western Blot: CGI-16 Antibody (4E4) [H00023597-M01] - ACATE2 monoclonal antibody (M01), clone 4E4 Analysis of ACATE2 expression in MCF-7.
Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody (4E4) [H00023597-M01]

Immunocytochemistry/ Immunofluorescence: CGI-16 Antibody (4E4) [H00023597-M01]

Immunocytochemistry/Immunofluorescence: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of monoclonal antibody to ACOT9 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: CGI-16 Antibody (4E4) [H00023597-M01]

Immunohistochemistry-Paraffin: CGI-16 Antibody (4E4) [H00023597-M01]

Immunohistochemistry-Paraffin: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Western Blot: CGI-16 Antibody (4E4) [H00023597-M01]

Western Blot: CGI-16 Antibody (4E4) [H00023597-M01]

Western Blot: CGI-16 Antibody (4E4) [H00023597-M01] - Analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody (M01), clone 4E4.Lane 1: ACOT9 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: CGI-16 Antibody (4E4) [H00023597-M01] - Detection limit for recombinant GST tagged ACOT9 is approximately 3ng/ml as a capture antibody.

Applications for CGI-16 Antibody (4E4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recominant protein and cell lysate for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CGI-16

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fattyacid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Active on long chain acyl-CoAs

Alternate Names

ACATE2, Acyl-CoA thioester hydrolase 9, acyl-CoA thioesterase 9acyl-Coenzyme A thioesterase 2, mitochondrial, acyl-coenzyme A thioesterase 9, mitochondrial, CGI-16, EC 3.1.2, EC 3.1.2.-, EC 3.1.2.15, mitochondrial Acyl-CoA Thioesterase, MT-ACT48

Entrez Gene IDs

23597 (Human)

Gene Symbol

ACOT9

UniProt

AAH12573 (Human)

Additional CGI-16 Products

Product Documents for CGI-16 Antibody (4E4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for CGI-16 Antibody (4E4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CGI-16 Antibody (4E4) - Azide and BSA Free

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Protocols

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