• Format
    96-well strip plate
  • Assay Length
    3.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Cell Lysates (100 uL), EDTA Plasma (100 uL), Saliva (100 uL), Urine (100 uL)
  • Sensitivity
    3.06 pmol/mL
  • Assay Range
    2.1 - 500 pmol/mL (Cell Culture Supernates, Cell Lysates, EDTA Plasma, Saliva, Urine)
  • Specificity
    Measures cyclic GMP levels in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Parameter cGMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cGMP in cell culture supernates, plasma, saliva, urine, and cell lysates.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Saliva, Urine
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.912.514816.522.4

Cell Lysates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation2.249.544.412.7


The recovery of cGMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 100 91-108
Cell Lysates (n=1) 92 80-98
EDTA Plasma (n=4) 91 74-105
Saliva (n=4) 102 80-123
Urine (n=4) 90 77-98
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cGMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
cGMP Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Background: cGMP
Guanosine 3, 5-cyclic monophosphate (cGMP) is formed via the action of guanylate cyclase on GTP. cGMP is present at levels typically 10-100 fold lower than cAMP in most tissues. A variety of stimuli including acetylcholine, insulin, oxytocin, serotonin, and histamine will cause an increase in cGMP levels. Various stimulators of guanylate cyclase (e.g. vasodilators) as well as peptides that relax smooth muscle also increase cGMP concentrations.
    • Long Name
      Cyclic GMP
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 150 µL Diluent to NSB wells & 100 µL Diluent to B0 wells
    4.   Add 150 µL of the appropriate diluent to the non-specific binding (NSB) wells and 100 µL of the appropriate diluent to the zero standard (B0) wells.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, Control, or sample to the remaining wells.

    7. 50 µL Conjugate
    8.   Add 50 µL of Conjugate to each well.

    9. 50 µL Primary Antibody Solution
    10.   Add 50 µL of Primary Antibody Solution to each well (except the NSB wells).
    11.   Cover with a plate sealer, and incubate at room temperature for 3 hours on a horizontal orbital microplate shaker.
    12.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    13. 200 µL Substrate Solution
    14.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    15. 50 µL Stop Solution
    16. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 10
    Filter your results:

    Sample Type
    1. S-palmitoylation of a Novel Site in the ?2-adrenergic Receptor Associated with a Novel Intracellular Itinerary
      J Biol Chem, 2016;0(0):.
      Species: Human
      Sample Type: Cell Lysates
    2. Simultaneous exposure to Escherichia coli heat-labile and heat-stable enterotoxins increases fluid secretion and alters cyclic nucleotide and cytokine production by intestinal epithelial cells.
      Authors: Read L, Hahn R, Thompson C, Bauer D, Norton E, Clements J
      Infect Immun, 2014;82(12):5308-16.
      Species: Human
      Sample Type: Cell Lysates
    3. Decompensated heart failure is associated with reduced corin levels and decreased cleavage of pro-atrial natriuretic peptide.
      Authors: Ibebuogu UN, Gladysheva IP, Houng AK
      Circ Heart Fail, 2011;4(2):114-20.
      Species: Human
      Sample Type: Plasma
    4. Thiophenecarboxylate suppressor of cyclic nucleotides discovered in a small-molecule screen blocks toxin-induced intestinal fluid secretion.
      Authors: Tradtrantip L, Yangthara B, Padmawar P, Morrison C, Verkman AS
      Mol. Pharmacol., 2009;75(1):134-42.
      Species: Hamster
      Sample Type: Cell Lysates
    5. Hematologic, biochemical, and cardiopulmonary effects of L-arginine supplementation or phosphodiesterase 5 inhibition in patients with sickle cell disease who are on hydroxyurea therapy.
      Authors: Little JA, Hauser KP, Martyr SE, Harris A, Maric I, Morris CR, Suh JH, Taylor J, Castro O, Machado R, Kato G, Gladwin MT
      Eur. J. Haematol., 2009;82(4):315-21.
      Species: Human
      Sample Type: Plasma
    6. Decreased nitric oxide products in the urine of patients undergoing cardiac surgery.
      Authors: Lema G, Urzua J, Jalil R, Canessa R, Vogel A, Moran S, Fajuri A, Carvajal C, Aeschlimann N, Jaque MP
      J. Cardiothorac. Vasc. Anesth., 2009;23(2):188-94.
      Species: Human
      Sample Type: Urine
    7. The role of interleukin-6 in UVA protection against UVB-induced immunosuppression.
      Authors: Reeve VE, Tyrrell RM, Allanson M, Domanski D, Blyth L
      J. Invest. Dermatol., 2009;129(6):1539-46.
      Species: Mouse
      Sample Type: Tissue Homogenates
    8. cGMP-independent anti-tumour actions of the inhibitor of soluble guanylyl cyclase, ODQ, in prostate cancer cell lines.
      Authors: Haramis G, Zhou Z, Pyriochou A, Koutsilieris M, Roussos C, Papapetropoulos A
      Br. J. Pharmacol., 2008;155(6):804-13.
      Species: Human
      Sample Type: Cell Culture Supernates
    9. Terfenadine-induced apoptosis in human melanoma cells is mediated through Ca2+ homeostasis modulation and tyrosine kinase activity, independently of H1 histamine receptors.
      Authors: Jangi SM, Ruiz-Larrea MB, Nicolau-Galmes F, Andollo N, Arroyo-Berdugo Y, Ortega-Martinez I, Diaz-Perez JL, Boyano MD
      Carcinogenesis, 2008;29(3):500-9.
      Species: Human
      Sample Type: Cell Lysates
    10. Successive action of meprin A and neprilysin catabolizes B-type natriuretic peptide.
      Authors: Pankow K, Wang Y, Gembardt F, Krause E, Sun X, Krause G, Schultheiss HP, Siems WE, Walther T
      Circ. Res., 2007;101(9):875-82.
      Species: Human
      Sample Type: Cell Lysates
    ELISA Controls
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    Parameter Immunoassay Control Set 870 for cGMP

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