CHIR 99021
Tocris Bioscience | Catalog # TB4423-GMP
Key Product Details
Description
Product Description
CHIR 99021 synthesized to cGMP guidelines. Suitable for use as an ancillary reagent in the further manufacturing of cell therapies.
For more information about how CHIR 99021 may be used, please see our protocols: Transdifferentiating Fibroblasts into Neurons, Culturing Transcriptomically Distinct Pluripotent mESCs, Converting Fibroblasts into Cardiomyocytes (9C Cocktail), Human iPSC-derived Myoblasts, Reprogramming MEFs into Skeletal Muscle Progenitors, Highly Efficient Generation of CiPSCs from MEFs, Generation of Kidney Organoids from hPSCs,Culturing Transcriptomically Distinct Pluripotent mESCs.
Research use only CHIR 99021 also available.
Scientific Data Images for CHIR 99021
iPSC-derived Human Heart-forming Organoids
Human iPSCs were embedded in Cultrex™ UltiMatrix RGF Basement Membrane Extract (R&D Systems, Catalog # BME001-05) and differentiated with CHIR 99021 (Tocris Bioscience, Catalog # TB4423-GMP) and IWP 2 (Tocris Bioscience, Catalog # 3533) into heart-forming organoids based on a protocol from Drakhlis, L. et al. (2021) Nat. Biotechnol. 39:737. Successful differentiation was demonstrated by staining the cells with A) a Rabbit Anti-Human Cardiac Troponin T Antigen Affinity-purified Polyclonal Antibody (green; Novus Biologicals, Catalog # NBP3-13391) to detect cardiomyocytes and a Goat Anti-Human CD31/PECAM-1 Antigen Affinity-purified Polyclonal Antibody (red; R&D Systems, Catalog # AF3628) to detect endothelial cells. The cells were counterstained with DAPI (blue; Tocris Bioscience, Catalog # 5748). As formation of early embryonic heart anlagen is associated with nascent foregut endoderm development, cells were also stained with B) a Goat Anti-Human SOX2 Antigen Affinity-purified Polyclonal Antibody (red; R&D Systems, Catalog # AF2018) and a Mouse Anti-Human HNF-4 alpha/NR2A1 Monoclonal Antibody (green; R&D Systems, Catalog # PP-K9218-00) to detect anterior and posterior foregut endoderm cells, respectively. The cells were counterstained with DAPI (blue; Tocris Bioscience, Catalog # 5748).
Current Good Manufacturing Practice (cGMP) is a harmonized set of guidelines that when implemented will ensure safe, reliable, consistent and quality-assured products. The Tocris range of cGMP small molecules is manufactured following relevant sections of ICH Q7 guidelines. Our highly skilled team has full control over the cGMP process, meaning we can ensure: a quality-assured manufacturing system, batch-to-batch consistency, consistent supply, traceability from starting material to final product, and animal-free production. Find out more about the Tocris range of cGMP compounds.
Product Specifications for CHIR 99021
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The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
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Background References
References are publications that support the biological activity of the product. See our Citations tab to view 4 publications citing the usage of this product.
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Product Documents for CHIR 99021
Certificate of Analysis
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Product Specific Notices for CHIR 99021
For preclinical, or clinical ex vivo use
Related Research Areas
Citations for CHIR 99021
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