Catalog # Availability Size / Price Qty
IWP 2 | CAS No. 686770-61-6 | PORCN Inhibitors
1 Image
Description: PORCN inhibitor; inhibits Wnt processing and secretion

Chemical Name: N-(6-Methyl-2-benzothiazolyl)-2-[(3,4,6,7-tetrahydro-4-oxo-3-phenylthieno[3,2-d]pyrimidin-2-yl)thio]-acetamide

Purity: ≥98%

Product Details
Citations (38)
Reviews (1)

Biological Activity

IWP 2 is a potent inhibitor of Wnt processing and secretion (IC50 = 27nM). IWP 2 inactivates PORCN, a membrane-bound O-acyltransferase (MBOAT), and selectively inhibits palmitoylation of Wnt. Blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. IWP 2 suppresses self-renewal in R1 embryonic stem cells and promotes cardiomyocyte differentiation from hPSCs. The compound has also been used in protocols to reprogram somatic cells to chemically-induced PSCs.

Technical Data

Soluble to 5 mM in DMSO with gentle warming
Store at +4°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Citations for IWP 2

The citations listed below are publications that use Tocris products. Selected citations for IWP 2 include:

38 Citations: Showing 1 - 10

  1. A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
    Authors: Yang Et al.
    Cell Stem Cell  2020;27:125
  2. Long-term functional maintenance of primary human hepatocytes in vitro.
    Authors: Xiang Et al.
    Science  2019;364:399
  3. In Vivo Generation of Post-infarct Human Cardiac Muscle by Laminin-Promoted Cardiovascular Progenitors.
    Authors: Yap Et al.
    Cell Rep  2019;26:3231
  4. User-Friendly and Parallelized Generation of Human Induced Pluripotent Stem Cell-Derived Microtissues in a Centrifugal Heart-on-a-Chip.
    Authors: Schneider Et al.
    Tissue Eng Part A  2019;25:786
  5. Point mutations in the PDX1 transactivation domain impair human β-cell development and function.
    Authors: Wang Et al.
    Mol Metab  2019;24:80
  6. Establishment of A Protocol for In Vitro Culture of Cardiogenic Mesodermal Cells Derived from Human Embryonic Stem Cells.
    Authors: Vahdat Et al.
    Cell J  2019;20:496
  7. TMEM88 Inhibits Wnt Signaling by Promoting Wnt Signalosome Localization to Multivesicular Bodies.
    Authors: Lee and Evans
    iScience  2019;19:267
  8. Capacitation of human na�ve pluripotent stem cells for multi-lineage differentiation.
    Authors: Rostovskaya Et al.
    Development  2019;146
  9. Geometrical Patterning and Constituent Cell Heterogeneity Facilitate Electrical Conduction Disturbances in a Human Induced Pluripotent Stem Cell-Based Platform: An In vitro Disease Model of Atrial Arrhythmias.
    Authors: Nakanishi Et al.
    Front Physiol  2019;10:818
  10. Temporal Regulation of Natural Killer T Cell Interferon Gamma Responses by β-Catenin-Dependent and -Independent Wnt Signaling.
    Authors: Kling Et al.
    Front Immunol  2018;9:483
  11. Effects of lung and airway epithelial maturation cocktail on the structure of lung bud organoids.
    Authors: Magro-Lopez Et al.
    Stem Cell Res.Ther.  2018;9:186
  12. ETV1 activates a rapid conduction transcriptional program in rodent and human cardiomyocytes.
    Authors: Shekhar Et al.
    Sci Rep  2018;8:9944
  13. Efficient differentiation of cardiomyocytes and generation of calcium-sensor reporter lines from nonhuman primate iPSCs.
    Authors: Lin Et al.
    Sci Rep  2018;8:5907
  14. Culture in Glucose-Depleted Medium Supplemented with Fatty Acid and 3,3',5-Triiodo-l-Thyronine Facilitates Purification and Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes.
    Authors: Lin Et al.
    Front Endocrinol (Lausanne)  2017;8:253
  15. A genome-wide screen identifies YAP/WBP2 interplay conferring growth advantage on human epidermal stem cells.
    Authors: Walko Et al.
    Nat Commun  2017;8:14744
  16. Human Pluripotent Stem Cell-Derived Atrial and Ventricular Cardiomyocytes Develop from Distinct Mesoderm Populations.
    Authors: Lee Et al.
    Cell Stem Cell  2017;21:179
  17. Degree of tissue differentiation dictates susceptibility to BRAF-driven colorectal cancer.
    Authors: Tong
    Cell Rep  2017;21(13):3833
  18. Genome Editing in hPSCs Reveals GATA6 Haploinsufficiency and a Genetic Interaction with GATA4 in Human Pancreatic Development.
    Authors: Shi Et al.
    Cell Stem Cell  2017;20:675
  19. Label-free imaging of metabolism and oxidative stress in human induced pluripotent stem cell-derived cardiomyocytes.
    Authors: Datta Et al.
    Biomed Opt Express  2016;7:1690
  20. Natural underlying mtDNA heteroplasmy as a potential source of intra-person hiPSC variability.
    Authors: Perales-Clemente Et al.
    EMBO J  2016;35:1979
  21. Regulation of WNT Signaling by VSX2 During Optic Vesicle Patterning in Human Induced Pluripotent Stem Cells.
    Authors: Capowski Et al.
    Stem Cells  2016;34:2625
  22. Wnt/β-catenin signaling promotes self-renewal and inhibits the primed state transition in na�ve human embryonic stem cells.
    Authors: Xu Et al.
    Proc Natl Acad Sci U S A  2016;113:E6382
  23. Sp5 and Sp8 recruit β-catenin and Tcf1-Lef1 to select enhancers to activate Wnt target gene transcription.
    Authors: Kennedy Et al.
    Proc Natl Acad Sci U S A  2016;113:3545
  24. HP Promotes Cardiac Differentiation of Human Pluripotent Stem Cells in Chemically Defined Albumin-Free Medium, Enabling Consistent Manufacture of Cardiomyocytes.
    Authors: Lin Et al.
    Stem Cells Transl Med  2016;
  25. Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids.
    Authors: Freedman Et al.
    Nat Cell Biol  2015;6:8715
  26. Machine learning plus optical flow: a simple and sensitive method to detect cardioactive drugs.
    Authors: Lee Et al.
    Sci Rep  2015;5:11817
  27. A systems-biological study on the identification of safe and effective molecular targets for the reduction of ultraviolet B-induced skin pigmentation.
    Authors: Lee Et al.
    Nat Commun  2015;5:10305
  28. Generation of an expandable intermediate mesoderm restricted progenitor cell line from human pluripotent stem cells.
    Authors: Kumar Et al.
    Elife  2015;4
  29. Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales.
    Authors: Huebsch Et al.
    Tissue Eng Part C Methods  2015;21:467
  30. Human definitive haemogenic endothelium and arterial vascular endothelium represent distinct lineages.
    Authors: Ditadi Et al.
    Stem Cell Res  2015;17:580
  31. Supervised Machine Learning for Classification of the Electrophysiological Effects of Chronotropic Drugs on Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
    Authors: Heylman Et al.
    PLoS One  2015;10:e0144572
  32. Act.-A and Bmp4 levels modulate cell type specification during CHIR-induced cardiomyogenesis.
    Authors: Kim Et al.
    PLoS One  2015;10:e0118670
  33. The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells.
    Authors: Huang Et al.
    Sci Rep  2015;10:413
  34. Wnt/β-catenin signaling modulates human airway sensitization induced by β2-adrenoceptor stimulation.
    Authors: Faisy Et al.
    PLoS One  2014;9:e111350
  35. Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture.
    Authors: Kempf Et al.
    Stem Cell Reports  2014;3:1132
  36. Efficient generation of lung and airway epithelial cells from human pluripotent stem cells.
    Authors: Huang Et al.
    Nat Biotechnol  2014;32:84
  37. SIRPA, VCAM1 and CD34 identify discrete lineages during early human cardiovascular development.
    Authors: Skelton Et al.
    Nat Genet  2014;13:172
  38. Hedgehog-Gli activators direct osteo-chondrogenic function of bone morphogenetic protein toward osteogenesis in the perichondrium.
    Authors: Hojo Et al.
    J Biol Chem  2013;288:9924


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Wnt pathway inhibitor that works really well
By Anonymous on 12/09/2019
Species: Human

I used it as a Wnt pathway inhibitor in Hepatocellular Carcinoma Cells. I used the concentration 5uM and it works really well.

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