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Human/Mouse/Rat SOX2 Antibody

AF2018 87 Citations 3 Reviews Datasheet / COA / SDS
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AF2018
AF2018-SP
Human/Mouse/Rat SOX2 Antibody in Western Blot
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Citations (87)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat SOX2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human SOX2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SOX17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human SOX2
Gly135-Met317
Accession # P48431
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
5 µg/mL
See below
Chromatin Immunoprecipitation (ChIP)
5 µg/5 x 106 cells
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat SOX2 by Western Blot. View Larger

Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, NTera-2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Chromatin Immunoprecipitation (ChIP) Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation. View Larger

Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 µg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 µL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. Thenanogpromoter was detected by standard PCR.

Immunocytochemistry SOX2 in Mouse Cortical Stem Cells. View Larger

SOX2 in Mouse Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry SOX2 in Rat Cortical Stem Cells. View Larger

SOX2 in Rat Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Simple Western Detection of Human SOX2 by Simple Western<sup>TM</sup>. View Larger

Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera-2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/ Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry SOX2 in ADLF1 and FAB2 Stem Cell Lines. View Larger

SOX2 in ADLF1 and FAB2 Stem Cell Lines. SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOX2

SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).

References
  1. Graham, V. et al. (2003) Neuron 39:749.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
  3. Kishi, M. et al. (2000) Development 127:791.
  4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
  5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
  6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
Long Name
Transcription Factor SOX2
Entrez Gene IDs
6657 (Human)
Alternate Names
ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2

Product Datasheets

Product Datasheet
COA
SDS

Citations for Human/Mouse/Rat SOX2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

87 Citations: Showing 1 - 10
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  1. Establishment of induced pluripotent stem cell line (GSPHi001-A) from a 6-year old female with nephrotic syndrome
    Authors: X Gao, X Gao, H Zhao, W Cui, M Tan, H Deng
    Stem Cell Res, 2019;41(0):101661.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels
    Authors: MA Skylar-Sco, SGM Uzel, LL Nam, JH Ahrens, RL Truby, S Damaraju, JA Lewis
    Sci Adv, 2019;5(9):eaaw2459.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. O-GlcNAc transferase activates stem-like cell potential in hepatocarcinoma through O-GlcNAcylation of eukaryotic initiation factor 4E
    Authors: B Cao, M Duan, Y Xing, C Liu, F Yang, Y Li, T Yang, Y Wei, Q Gao, J Jiang
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Chi3l3 induces oligodendrogenesis in an experimental model of autoimmune neuroinflammation
    Authors: SC Starossom, J Campo Garc, T Woelfle, S Romero-Sua, M Olah, F Watanabe, L Cao, A Yeste, JJ Tukker, FJ Quintana, J Imitola, F Witzel, D Schmitz, M Morkel, F Paul, C Infante-Du, SJ Khoury
    Nat Commun, 2019;10(1):217.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. The BAF and PRC2 Complex Subunits Dpf2 and Eed Antagonistically Converge on Tbx3 to Control ESC Differentiation
    Authors: W Zhang, C Chronis, X Chen, H Zhang, R Spalinskas, M Pardo, L Chen, G Wu, Z Zhu, Y Yu, L Yu, J Choudhary, J Nichols, MM Parast, B Greber, P Sahlén, K Plath
    Cell Stem Cell, 2019;24(1):138-152.e8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. YAP Activity Is Necessary and Sufficient for Basal Progenitor Abundance and Proliferation in the Developing Neocortex
    Authors: M Kostic, JTML Paridaen, KR Long, N Kalebic, B Langen, N Grübling, P Wimberger, H Kawasaki, T Namba, WB Huttner
    Cell Rep, 2019;27(4):1103-1118.e6.
    Species: Ferret
    Sample Types: Whole Tissue
    Applications: IHC
  7. Lack of whey acidic protein four disulphide core (WFDC) 2 protease inhibitor causes neonatal death from respiratory failure in mice
    Authors: K Nakajima, M Ono, U Radovi?, S Dizdarevi?, SI Tomizawa, K Kuroha, G Naganatsu, I Hoshi, R Matsunaga, T Shirakawa, T Kurosawa, Y Miyazaki, M Seki, Y Suzuki, H Koseki, M Nakamura, T Suda, K Ohbo
    Dis Model Mech, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  8. Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids
    Authors: YP Xu, Y Qiu, B Zhang, G Chen, Q Chen, M Wang, F Mo, J Xu, J Wu, RR Zhang, ML Cheng, NN Zhang, B Lyu, WL Zhu, MH Wu, Q Ye, D Zhang, JH Man, XF Li, J Cui, Z Xu, B Hu, X Zhou, CF Qin
    Cell Res., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Single-cell analysis reveals T cell infiltration in old neurogenic niches
    Authors: BW Dulken, MT Buckley, P Navarro Ne, N Saligrama, R Cayrol, DS Leeman, BM George, SC Boutet, K Hebestreit, JV Pluvinage, T Wyss-Coray, IL Weissman, H Vogel, MM Davis, A Brunet
    Nature, 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis
    Authors: B Di Stefano, EC Luo, C Haggerty, S Aigner, J Charlton, J Brumbaugh, F Ji, I Rabano Jim, KJ Clowers, AJ Huebner, K Clement, I Lipchina, MAC de Kort, A Anselmo, J Pulice, MFM Gerli, H Gu, SP Gygi, RI Sadreyev, A Meissner, GW Yeo, K Hochedling
    Cell Stem Cell, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  11. Epigenomic Profiling Discovers Trans-lineage SOX2 Partnerships Driving Tumor Heterogeneity in Lung Squamous Cell Carcinoma
    Authors: T Sato, S Yoo, R Kong, A Sinha, P Chandraman, A Patel, M Fridrikh, O Nagano, T Masuko, MB Beasley, CA Powell, J Zhu, H Watanabe
    Cancer Res., 2019;79(24):6084-6100.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  12. Lysine acetyltransferase 8 is involved in cerebral development and syndromic intellectual disability
    Authors: L Li, M Ghorbani, M Weisz-Hubs, J Rousseau, I Thiffault, RE Schnur, C Breen, R Oegema, MM Weiss, Q Waisfisz, S Welner, H Kingston, JA Hills, EM Boon, L Basel-Salm, O Konen, H Goldberg-S, L Bazak, S Tzur, J Jin, X Bi, M Bruccoleri, K McWalter, MT Cho, M Scarano, GB Schaefer, SS Brooks, SS Hughes, KLI van Gassen, JM van Hagen, TK Pandita, PB Agrawal, PM Campeau, XJ Yang
    J. Clin. Invest., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Gastrointestinal transcription factors drive lineage-specific developmental programs in organ specification and cancer
    Authors: R Francis, H Guo, C Streutker, M Ahmed, T Yung, PB Dirks, HH He, TH Kim
    Sci Adv, 2019;5(12):eaax8898.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  14. BMP and WNT signalling cooperate through LEF1 in the neuronal specification of adult hippocampal neural stem and progenitor cells
    Authors: T Armenteros, Z Andreu, R Hortigüela, DC Lie, H Mira
    Sci Rep, 2018;8(1):9241.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  15. Submucosal Gland Myoepithelial Cells Are Reserve Stem Cells That Can Regenerate Mouse Tracheal Epithelium
    Authors: TJ Lynch, PJ Anderson, PG Rotti, SR Tyler, AK Crooke, SH Choi, DT Montoro, CL Silverman, W Shahin, R Zhao, CW Jensen-Cod, A Adamcakova, TIA Evans, W Xie, Y Zhang, H Mou, BP Herring, PS Thorne, J Rajagopal, C Yeaman, KR Parekh, JF Engelhardt
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. BCL11A interacts with SOX2 to control the expression of epigenetic regulators in lung squamous carcinoma
    Authors: KA Lazarus, F Hadi, E Zambon, K Bach, MF Santolla, JK Watson, LL Correia, M Das, R Ugur, S Pensa, L Becker, LS Campos, G Ladds, P Liu, GI Evan, FM McCaughan, J Le Quesne, JH Lee, D Calado, WT Khaled
    Nat Commun, 2018;9(1):3327.
    Species: Human
    Sample Types: DNA
    Applications: ChIP
  17. Generation of a human Juvenile myelomonocytic leukemia iPSC line, CHOPi001-A, with a mutation in CBL
    Authors: AL Gagne, JA Maguire, S Gandre-Bab, ST Chou, SK Tasian, ML Loh, MJ Weiss, P Gadue, DL French
    Stem Cell Res, 2018;31(0):157-160.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  18. Selective enrichment of CD133+/SOX2+ glioblastoma stem cells via adherent culture
    Authors: K Lv, Z Chen, X Zhang, Q Zhang, L Liu
    Oncol Lett, 2018;16(4):4567-4576.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  19. Treatment of Human Glioblastoma with a Live Attenuated Zika Virus Vaccine Candidate
    Authors: Q Chen, J Wu, Q Ye, F Ma, Q Zhu, Y Wu, C Shan, X Xie, D Li, X Zhan, C Li, XF Li, X Qin, T Zhao, H Wu, PY Shi, J Man, CF Qin
    MBio, 2018;9(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Transcriptional repressor GATA binding 1-mediated repression of SRY-box 2 expression suppresses cancer stem cell functions and tumor initiation
    Authors: X Gong, W Liu, L Wu, Z Ma, Y Wang, S Yu, J Zhang, H Xie, G Wei, F Ma, L Lu, L Chen
    J. Biol. Chem., 2018;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  21. Generation of seven induced pluripotent stem cell lines from neonates of different ethnic backgrounds
    Authors: Y Yin, AJ Petersen, C Soref, WD Richards, T Ludwig, S Taapken, E Berndt, SC Zhang, A Bhattachar
    Stem Cell Res, 2018;34(0):101365.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  22. Generation of Spinocerebellar Ataxia Type 2 induced pluripotent stem cell lines, CHOPi002-A and CHOPi003-A, from patients with abnormal CAG repeats in the coding region of the ATXN2 gene
    Authors: JA Maguire, AL Gagne, P Gonzalez-A, BL Davidson, V Shakkottai, P Gadue, DL French
    Stem Cell Res, 2018;34(0):101361.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  23. NADsupplementation normalizes key Alzheimer's features and DNA damage responses in a new AD mouse model with introduced DNA repair deficiency
    Authors: Y Hou, S Lautrup, S Cordonnier, Y Wang, DL Croteau, E Zavala, Y Zhang, K Moritoh, JF O'Connell, BA Baptiste, TV Stevnsner, MP Mattson, VA Bohr
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  24. NMDA receptor signaling is important for neural tube formation and for preventing antiepileptic drug-induced neural tube defects
    Authors: EB Sequerra, R Goyal, PA Castro, JB Levin, LN Borodinsky
    J. Neurosci., 2018;0(0):.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC
  25. Developmental History Provides a Roadmap for the Emergence of Tumor Plasticity
    Authors: PR Tata, RD Chow, SV Saladi, A Tata, A Konkimalla, A Bara, D Montoro, LP Hariri, AR Shih, M Mino-Kenud, H Mou, S Kimura, LW Ellisen, J Rajagopal
    Dev. Cell, 2018;44(6):679-693.e5.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Central and Peripheral Nervous System Progenitors Derived from Human Pluripotent Stem Cells Reveal a Unique Temporal and Cell-Type Specific Expression of PMCAs
    Authors: M Chen, SH Laursen, M Habekost, CH Knudsen, SH Buchholdt, J Huang, F Xu, X Liu, L Bolund, Y Luo, P Nissen, F Febbraro, M Denham
    Front Cell Dev Biol, 2018;6(0):5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  27. Complementary expression of calcium binding proteins delineates the functional organization of the locomotor network
    Authors: EM Berg, M Bertuzzi, K Ampatzis
    Brain Struct Funct, 2018;0(0):.
    Species: Zebrafish
    Sample Types: Whole Tissue
    Applications: IHC
  28. SNAI2 upregulation is associated with an aggressive phenotype in fulvestrant-resistant breast cancer cells and is an indicator of poor response to endocrine therapy in estrogen receptor-positive metastatic breast cancer
    Authors: CL Alves, D Elias, MB Lyng, M Bak, HJ Ditzel
    Breast Cancer Res., 2018;20(1):60.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  29. Evidence of functional duplicity of Nestin expression in the adult mouse midbrain
    Authors: P Farzanehfa, SS Lu, A Dey, D Musiienko, H Baagil, MK Horne, TD Aumann
    Stem Cell Res, 2017;19(0):82-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Sox2 regulates M�ller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a
    Authors: RA Gorsuch, M Lahne, CE Yarka, ME Petravick, J Li, DR Hyde
    Exp. Eye Res., 2017;0(0):.
    Species: Fish
    Sample Types: Whole Tissue
    Applications: IHC
  31. Synaptic regulator ?-synuclein in dopaminergic fibers is essentially required for the maintenance of subependymal neural stem cells
    Authors: A Perez-Vill, M Salomé Sir, G Belenguer, R Soriano-Ca, A Belén Muño, J Villadiego, D Alarcón-Ar, FN Soria, B Dehay, E Bezard, M Vila, A Bortolozzi, J José Toled, F Pérez-Sánc, I Fariñas
    J. Neurosci., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts
    Authors: CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  33. Directed transdifferentiation of M�ller glial cells to photoreceptors using the sonic hedgehog signaling pathway agonist purmorphamine
    Authors: D Gu, S Wang, S Zhang, P Zhang, G Zhou
    Mol Med Rep, 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  34. Sin3a regulates epithelial progenitor cell fate during lung development
    Authors: C Yao, G Carraro, B Konda, X Guan, T Mizuno, N Chiba, M Kostelny, A Kurkciyan, G David, JL McQualter, BR Stripp
    Development, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Generation of a multipurpose Prdm16 allele by targeted trapping
    Authors: A Strassman, F Schnütgen, Q Dai, JC Jones, AC Gomez, L Pitstick, NE Holton, R Moskal, ER Leslie, H von Melchn, DR Beier, BC Bjork
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  36. Human neural progenitors derived from integration-free iPSCs for SCI therapy
    Authors: Y Liu, Y Zheng, S Li, H Xue, K Schmitt, GW Hergenroed, J Wu, Y Zhang, DH Kim, Q Cao
    Stem Cell Res, 2017;19(0):55-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  37. Folate receptor alpha is necessary for neural plate cell apical constriction during Xenopus neural tube formation
    Authors: OA Balashova, O Visina, LN Borodinsky
    Development, 2017;0(0):.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-P
  38. Long-Distance Axonal Growth and Protracted Functional Maturation of Neurons Derived From Human Induced Pluripotent Stem Cells After Intracerebral Transplantation
    Authors: JC Niclis, C Turner, J Durnall, S McDougal, JA Kauhausen, B Leaw, M Dottori, CL Parish, LH Thompson
    Stem Cells Transl Med, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells
    Authors: IL da Silva, L Montero-Mo, E Martín-Vil, J Martin-Pér, B Sainz, J Renart, R Toscano Si, É Soares Vel, C Salviano T, MC de Oliveir, E Ferreira, M Quintanill
    Sci Rep, 2017;7(1):6276.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  40. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  41. Peptide-Based Scaffolds Support Human Cortical Progenitor Graft Integration to Reduce Atrophy and Promote Functional Repair in a Model of Stroke
    Authors: FA Somaa, TY Wang, JC Niclis, KF Bruggeman, JA Kauhausen, H Guo, S McDougall, RJ Williams, DR Nisbet, LH Thompson, CL Parish
    Cell Rep, 2017;20(8):1964-1977.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  42. Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
    Authors: J Belkind-Ge, HK Graham, J Reynolds, R Hotta, N Nagy, L Cheng, M Kamionek, HN Shi, CM Aherne, AM Goldstein
    Sci Rep, 2017;7(1):2525.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  43. Patient-Specific Screening Using High-Grade Glioma Explants to Determine Potential Radiosensitization by a TGF-? Small Molecule Inhibitor
    Neoplasia, 2016;18(12):795-805.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  44. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation
    Authors: Qi Xie
    J Clin Invest, 2016;126(7):2757-2772.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  45. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
    PLoS Biol., 2016;14(11):e1002581.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  46. An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals
    Sci Rep, 2016;6(0):29578.
    Species: Ferret
    Sample Types: Whole Tissue
    Applications: IHC
  47. Traumatic Brain Injury Stimulates Neural Stem Cell Proliferation via Mammalian Target of Rapamycin Signaling Pathway Activation
    Authors: Jinhui Chen
    eNeuro, 2016;3(5):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  48. Increased cycling cell numbers and stem cell associated proteins as potential biomarkers for high grade human papillomavirus+ve pre-neoplastic cervical disease.
    Authors: Canham, Maurice, Charsou, Chara, Stewart, June, Moncur, Sharon, Hoodless, Laura, Bhatia, Ramya, Cong, Duanduan, Cubie, Heather, Busby-Earle, Camille, Williams, Alistair, McLoughlin, Victoria, Campbell, John D M, Cuschieri, Kate, Howie, Sarah
    PLoS ONE, 2015;9(12):e115379.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  49. Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists.
    Authors: Takahashi, Teruaki, Takase, Yuta, Yoshino, Takashi, Saito, Daisuke, Tadokoro, Ryosuke, Takahashi, Yoshiko
    PLoS ONE, 2015;10(1):e0116119.
    Species: Avian - Quail
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  50. DNMT-dependent suppression of microRNA regulates the induction of GBM tumor-propagating phenotype by Oct4 and Sox2.
    Authors: Lopez-Bertoni H, Lal B, Li A, Caplan M, Guerrero-Cazares H, Eberhart C, Quinones-Hinojosa A, Glas M, Scheffler B, Laterra J, Li Y
    Oncogene, 2015;34(30):3994-4004.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  51. Deficiency of the chromatin regulator BRPF1 causes abnormal brain development.
    Authors: You L, Zou J, Zhao H, Bertos N, Park M, Wang E, Yang X
    J Biol Chem, 2015;290(11):7114-29.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  52. Mitochondrial control by DRP1 in brain tumor initiating cells.
    Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
    Nat Neurosci, 2015;18(4):501-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  53. The chromatin regulator Brpf1 regulates embryo development and cell proliferation.
    Authors: You L, Yan K, Zou J, Zhao H, Bertos N, Park M, Wang E, Yang X
    J Biol Chem, 2015;290(18):11349-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  54. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015;4(3):419-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  55. MOF-associated complexes ensure stem cell identity and Xist repression.
    Authors: Chelmicki T, Dundar F, Turley M, Khanam T, Aktas T, Ramirez F, Gendrel A, Wright P, Videm P, Backofen R, Heard E, Manke T, Akhtar A
    Elife, 2014;3(0):e02024.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  56. Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
    Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
    PLoS ONE, 2014;9(2):e88505.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  57. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system.
    Authors: Lee, Kyung Eu, Seo, Jihae, Shin, Jiheon, Ji, Eun Hye, Roh, Jiwon, Kim, Joo Yeon, Sun, Woong, Muhr, Jonas, Lee, Sanghyuk, Kim, Jaesang
    Proc Natl Acad Sci U S A, 2014;111(7):2794-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  58. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
    Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
    PLoS ONE, 2014;9(12):e116114.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  59. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
    Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James
    PLoS Biol, 2014;12(8):e1001937.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  60. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2014;141(2):377-88.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  61. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
    Authors: Ferrarese R, Harsh G, Yadav A, Bug E, Maticzka D, Reichardt W, Dombrowski S, Miller T, Masilamani A, Dai F, Kim H, Hadler M, Scholtens D, Yu I, Beck J, Srinivasasainagendra V, Costa F, Baxan N, Pfeifer D, von Elverfeldt D, Backofen R, Weyerbrock A, Duarte C, He X, Prinz M, Chandler J, Vogel H, Chakravarti A, Rich J, Carro M, Bredel M
    J Clin Invest, 2014;124(7):2861-76.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  62. Role of SOX2 in the etiology of embryonal carcinoma, based on analysis of the NCCIT and NT2 cell lines.
    Authors: Eini, Ronak, Stoop, Hans, Gillis, Ad J M, Biermann, Katharin, Dorssers, Lambert, Looijenga, Leendert
    PLoS ONE, 2014;9(1):e83585.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC-P
  63. Pharmacological inhibition of poly(ADP-ribose) polymerase-1 modulates resistance of human glioblastoma stem cells to temozolomide.
    Authors: Tentori, Lucio, Ricci-Vitiani, Lucia, Muzi, Alessia, Ciccarone, Fabio, Pelacchi, Federica, Calabrese, Roberta, Runci, Daniele, Pallini, Roberto, Caiafa, Paola, Graziani, Grazia
    BMC Cancer, 2014;14(0):151.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  64. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
    Authors: Schrode N, Saiz N, Di Talia S, Hadjantonakis A
    Dev Cell, 2014;29(4):454-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  65. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain.
    Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle
    Science, 2014;344(6183):1252304.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  66. 3D imaging of Sox2 enhancer clusters in embryonic stem cells.
    Authors: Liu, Zhe, Legant, Wesley R, Chen, Bi-Chang, Li, Li, Grimm, Jonathan, Lavis, Luke D, Betzig, Eric, Tjian, Robert
    Elife, 2014;3(0):e04236.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  67. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013;12(6):699-712.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ChIP
  68. Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth.
    Authors: Verginelli F, Perin A, Dali R, Fung K, Lo R, Longatti P, Guiot M, Del Maestro R, Rossi S, di Porzio U, Stechishin O, Weiss S, Stifani S
    Nat Commun, 2013;4(0):2956.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  69. SOX2 expression associates with stem cell state in human ovarian carcinoma.
    Authors: Bareiss, Petra M, Paczulla, Anna, Wang, Hui, Schairer, Rebekka, Wiehr, Stefan, Kohlhofer, Ursula, Rothfuss, Oliver C, Fischer, Anna, Perner, Sven, Staebler, Annette, Wallwiener, Diethelm, Fend, Falko, Fehm, Tanja, Pichler, Bernd, Kanz, Lothar, Quintanilla-Martinez, Leticia, Schulze-Osthoff, Klaus, Essmann, Frank, Lengerke, Claudia
    Cancer Res, 2013;73(17):5544-55.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  70. Efficient generation of human iPSCs by a synthetic self-replicative RNA.
    Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S
    Cell Stem Cell, 2013;13(2):246-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  71. BEND6 is a nuclear antagonist of Notch signaling during self-renewal of neural stem cells.
    Authors: Dai Q, Andreu-Agullo C, Insolera R, Wong L, Shi S, Lai E
    Development, 2013;140(9):1892-902.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  72. The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages.
    Authors: Sugiyama T, Osumi N, Katsuyama Y
    Dev Dyn, 2013;242(12):1442-53.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  73. Sox2 marks epithelial competence to generate teeth in mammals and reptiles.
    Authors: Juuri, Emma, Jussila, Maria, Seidel, Kerstin, Holmes, Scott, Wu, Ping, Richman, Joy, Heikinheimo, Kristiin, Chuong, Cheng-Mi, Arnold, Katrin, Hochedlinger, Konrad, Klein, Ophir, Michon, Frederic, Thesleff, Irma
    Development, 2013;140(7):1424-32.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  74. Sex determining region Y-box 2 (SOX2) amplification is an independent indicator of disease recurrence in sinonasal cancer.
    Authors: Schrock A, Goke F, Wagner P, Bode M, Franzen A, Braun M, Huss S, Agaimy A, Ihrler S, Menon R, Kirsten R, Kristiansen G, Bootz F, Lengerke C, Perner S
    PLoS ONE, 2013;8(3):e59201.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  75. Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord.
    Authors: Belgacem YH, Borodinsky LN,
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(11):4482-7.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC
  76. Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
    Authors: Engelen E, Akinci U, Bryne JC
    Nat. Genet., 2011;43(6):607-11.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  77. Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
    Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ
    Br. J. Cancer, 2011;105(6):854-63.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  78. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  79. Activity-dependent expression of Lmx1b regulates specification of serotonergic neurons modulating swimming behavior.
    Authors: Demarque M, Spitzer NC
    Neuron, 2010;67(2):321-34.
    Species: Xenopus
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  80. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Types: Cell Lysates
    Applications: ChIP
  81. Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.
    Authors: Pollard SM, Yoshikawa K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire JA, Smith A, Dirks P
    Cell Stem Cell, 2009;4(6):568-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  82. Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells.
    Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA
    Cancer Cell, 2008;13(1):69-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  83. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  84. Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells.
    Authors: Bourguignon LY, Peyrollier K, Xia W, Gilad E
    J. Biol. Chem., 2008;283(25):17635-51.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  85. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007;505(1):73-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  86. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007;448(7150):196-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  87. Microglia instruct subventricular zone neurogenesis.
    Authors: Walton NM, Sutter BM, Laywell ED, Levkoff LH, Kearns SM, Marshall GP, Scheffler B, Steindler DA
    Glia, 2006;54(8):815-25.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Reviews for Human/Mouse/Rat SOX2 Antibody

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Human/Mouse/Rat SOX2 Antibody
By Anonymous on 10/09/2019
Application: ICC/IF Sample Tested: Embryonic lung Species: Mouse

The sample (mouse embryonic lung) was fixed in 4% PFA for 1 hour and washed 3 times with PBS prior to embedding in OCT and cryosection. No antigen retrieval required. It labels the epithelial cells of bronchioles. Works Great!

Immunocytochemistry/Immunofluorescence Human/Mouse/Rat SOX2 Antibody AF2018
Human/Mouse/Rat SOX2 Antibody
By Anonymous on 01/25/2018
Application: WB Sample Tested: IPS2 induced pluripotent stem cells Species: Mouse
Western Blot Human/Mouse/Rat SOX2 Antibody AF2018
Human/Mouse/Rat SOX2 Antibody
By Anonymous on 11/23/2017
Application: IHC Sample Tested: Embryonic stem cells differentiated into dopaminergic neurons Species: Human
Immunohistochemistry Human/Mouse/Rat SOX2 Antibody AF2018