Human/Mouse/Rat SOX2 Antibody

(52 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects human SOX2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SOX1 and rhSOX17 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human SOX2
    Gly135-Met317
    Accession # P48431
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • Chromatin Immunoprecipitation (ChIP)
    5 µg/5 x 106 cells
    See below
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat SOX2 by Western Blot. Western blot shows lysates of D3 mouse embryonic stem cell line, NTera‑2 human testicular embryonic carcinoma cell line, F9 mouse teratocarcinoma stem cells, and rat cortical stem cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SOX2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Chromatin Immunoprecipitation (ChIP)
Detection of SOX2-regulated Genes by Chromatin Immunoprecipitation. BG01V human embryonic stem cells were fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. SOX2/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The nanog promoter was detected by standard PCR.
Immunocytochemistry
SOX2 in Mouse Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated mouse cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected in stem cells using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and co-stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Immunocytochemistry
SOX2 in Rat Cortical Stem Cells. SOX2 was detected in immersion fixed undifferentiated rat cortical stem cells using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Nestin was also detected using Mouse Anti-Mouse/Rat Nestin Monoclonal Antibody (Catalog # MAB2736) and stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009). Specific staining of SOX2 was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human SOX2 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells and NTera‑2 human testicular embryonic carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for SOX2 at approximately 53 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/
Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Immunocytochemistry
SOX2 in ADLF1 and FAB2 Stem Cell Lines. SOX2 was detected in immersion fixed ADLF1 (top panel) and FAB2 (bottom panel) induced pluripotent stem cell lines using Goat Anti-Human/Mouse/Rat SOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2018) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SOX2
SOX2 belongs to the SOX (SRY-like HMG box) family of transcription factors with diverse roles in development. SOX2 functions in specifying the first three lineages present at implantation and in regulating proliferation and differentiation in the developing peripheral nervous system (1-5).
  • References:
    1. Graham, V. et al. (2003) Neuron 39:749.
    2. Avilion, A.A. et al. (2003) Genes Dev. 17:126.
    3. Kishi, M. et al. (2000) Development 127:791.
    4. Yuan, H. et al. (1995) Genes Dev. 9:2635.
    5. Uwanogho, D. et al. (1995) Mech. Dev. 49:23.
    6. Stevanovic, M. (2003) Mol. Biol. Rep. 30:127.
  • Long Name:
    Transcription Factor SOX2
  • Entrez Gene IDs:
    6657 (Human)
  • Alternate Names:
    ANOP3; MCOPS3; MGC2413; SOX2; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

52 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Colitis promotes neuronal differentiation of Sox2+ and PLP1+ enteric cells
    Authors: J Belkind-Ge, HK Graham, J Reynolds, R Hotta, N Nagy, L Cheng, M Kamionek, HN Shi, CM Aherne, AM Goldstein
    Sci Rep, 2017;7(1):2525.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  2. Reduced expression of the murine HLA-G homolog Qa-2 is associated with malignancy, epithelial-mesenchymal transition and stemness in breast cancer cells
    Authors: IL da Silva, L Montero-Mo, E Martín-Vil, J Martin-Pér, B Sainz, J Renart, R Toscano Si, É Soares Vel, C Salviano T, MC de Oliveir, E Ferreira, M Quintanill
    Sci Rep, 2017;7(1):6276.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. Evidence of functional duplicity of Nestin expression in the adult mouse midbrain
    Authors: P Farzanehfa, SS Lu, A Dey, D Musiienko, H Baagil, MK Horne, TD Aumann
    Stem Cell Res, 2017;19(0):82-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  4. Generation of a multipurpose Prdm16 allele by targeted trapping
    Authors: A Strassman, F Schnütgen, Q Dai, JC Jones, AC Gomez, L Pitstick, NE Holton, R Moskal, ER Leslie, H von Melchn, DR Beier, BC Bjork
    Dis Model Mech, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  5. Human neural progenitors derived from integration-free iPSCs for SCI therapy
    Authors: Y Liu, Y Zheng, S Li, H Xue, K Schmitt, GW Hergenroed, J Wu, Y Zhang, DH Kim, Q Cao
    Stem Cell Res, 2017;19(0):55-64.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  6. Peptide-Based Scaffolds Support Human Cortical Progenitor Graft Integration to Reduce Atrophy and Promote Functional Repair in a Model of Stroke
    Authors: FA Somaa, TY Wang, JC Niclis, KF Bruggeman, JA Kauhausen, H Guo, S McDougall, RJ Williams, DR Nisbet, LH Thompson, CL Parish
    Cell Rep, 2017;20(8):1964-1977.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  7. CryoPause: A New Method to Immediately Initiate Experiments after Cryopreservation of Pluripotent Stem Cells
    Authors: KG Wong, SD Ryan, K Ramnarine, SA Rosen, SE Mann, A Kulick, E De Stanchi, FJ Müller, TJ Kacmarczyk, C Zhang, D Betel, MJ Tomishima
    Stem Cell Reports, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Succession of transiently active tumor-initiating cell clones in human pancreatic cancer xenografts
    Authors: CR Ball, F Oppel, KR Ehrenberg, TD Dubash, SM Dieter, CM Hoffmann, U Abel, F Herbst, M Koch, J Werner, F Bergmann, N Ishaque, M Schmidt, C von Kalle, C Scholl, S Fröhling, B Brors, W Weichert, J Weitz, H Glimm
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  9. Patient-Specific Screening Using High-Grade Glioma Explants to Determine Potential Radiosensitization by a TGF-? Small Molecule Inhibitor
    Neoplasia, 2016;18(12):795-805.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  10. An essential role of SVZ progenitors in cortical folding in gyrencephalic mammals
    Sci Rep, 2016;6(0):29578.
    Species: Ferret
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  11. RBPJ maintains brain tumor-initiating cells through CDK9-mediated transcriptional elongation
    Authors: Qi Xie
    J Clin Invest, 2016;126(7):2757-2772.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. SOX2 and PI3K Cooperate to Induce and Stabilize a Squamous-Committed Stem Cell Injury State during Lung Squamous Cell Carcinoma Pathogenesis
    PLoS Biol., 2016;14(11):e1002581.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  13. Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans.
    Authors: Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B, Bilodeau M, Sambi M, Rogers I, Rossant J, Bear C, Post M
    Stem Cell Reports, 2015;4(3):419-30.
    Species: Mouse
    Sample Type: Whole Cells
    Application: IHC Not Specified
  14. Increased cycling cell numbers and stem cell associated proteins as potential biomarkers for high grade human papillomavirus+ve pre-neoplastic cervical disease.
    Authors: Canham, Maurice, Charsou, Chara, Stewart, June, Moncur, Sharon, Hoodless, Laura, Bhatia, Ramya, Cong, Duanduan, Cubie, Heather, Busby-Earle, Camille, Williams, Alistair, McLoughlin, Victoria, Campbell, John D M, Cuschieri, Kate, Howie, Sarah
    PLoS ONE, 2015;9(12):e115379.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  15. The chromatin regulator Brpf1 regulates embryo development and cell proliferation.
    Authors: You L, Yan K, Zou J, Zhao H, Bertos N, Park M, Wang E, Yang X
    J Biol Chem, 2015;290(18):11349-64.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Angiogenesis in the developing spinal cord: blood vessel exclusion from neural progenitor region is mediated by VEGF and its antagonists.
    Authors: Takahashi, Teruaki, Takase, Yuta, Yoshino, Takashi, Saito, Daisuke, Tadokoro, Ryosuke, Takahashi, Yoshiko
    PLoS ONE, 2015;10(1):e0116119.
    Species: Avian - Quail
    Sample Type: Whole Tissue
    Application: IHC Frozen
  17. Deficiency of the chromatin regulator BRPF1 causes abnormal brain development.
    Authors: You L, Zou J, Zhao H, Bertos N, Park M, Wang E, Yang X
    J Biol Chem, 2015;290(11):7114-29.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  18. Mitochondrial control by DRP1 in brain tumor initiating cells.
    Authors: Xie Q, Wu Q, Horbinski C, Flavahan W, Yang K, Zhou W, Dombrowski S, Huang Z, Fang X, Shi Y, Ferguson A, Kashatus D, Bao S, Rich J
    Nat Neurosci, 2015;18(4):501-10.
    Species: Human
    Sample Type: Whole Tissue
    Application: IF
  19. DNMT-dependent suppression of microRNA regulates the induction of GBM tumor-propagating phenotype by Oct4 and Sox2.
    Authors: Lopez-Bertoni H, Lal B, Li A, Caplan M, Guerrero-Cazares H, Eberhart C, Quinones-Hinojosa A, Glas M, Scheffler B, Laterra J, Li Y
    Oncogene, 2015;34(30):3994-4004.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  20. GATA6 levels modulate primitive endoderm cell fate choice and timing in the mouse blastocyst.
    Authors: Schrode N, Saiz N, Di Talia S, Hadjantonakis A
    Dev Cell, 2014;29(4):454-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  21. Pharmacological inhibition of poly(ADP-ribose) polymerase-1 modulates resistance of human glioblastoma stem cells to temozolomide.
    Authors: Tentori, Lucio, Ricci-Vitiani, Lucia, Muzi, Alessia, Ciccarone, Fabio, Pelacchi, Federica, Calabrese, Roberta, Runci, Daniele, Pallini, Roberto, Caiafa, Paola, Graziani, Grazia
    BMC Cancer, 2014;14(0):151.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  22. Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
    Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
    Development, 2014;141(2):377-88.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Not Specified
  23. Expression and regulation of prostate apoptosis response-4 (Par-4) in human glioma stem cells in drug-induced apoptosis.
    Authors: Jagtap, Jayashre, Dawood, Parveen, Shah, Reecha D, Chandrika, Goparaju, Natesh, Kumar, Shiras, Anjali, Hegde, Amba S, Ranade, Deepak, Shastry, Padma
    PLoS ONE, 2014;9(2):e88505.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  24. Lineage-specific splicing of a brain-enriched alternative exon promotes glioblastoma progression.
    Authors: Ferrarese R, Harsh G, Yadav A, Bug E, Maticzka D, Reichardt W, Dombrowski S, Miller T, Masilamani A, Dai F, Kim H, Hadler M, Scholtens D, Yu I, Beck J, Srinivasasainagendra V, Costa F, Baxan N, Pfeifer D, von Elverfeldt D, Backofen R, Weyerbrock A, Duarte C, He X, Prinz M, Chandler J, Vogel H, Chakravarti A, Rich J, Carro M, Bredel M
    J Clin Invest, 2014;124(7):2861-76.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  25. Neuronal activity promotes oligodendrogenesis and adaptive myelination in the mammalian brain.
    Authors: Gibson, Erin M, Purger, David, Mount, Christop, Goldstein, Andrea K, Lin, Grant L, Wood, Lauren S, Inema, Ingrid, Miller, Sarah E, Bieri, Gregor, Zuchero, J Bradle, Barres, Ben A, Woo, Pamelyn, Vogel, Hannes, Monje, Michelle
    Science, 2014;344(6183):1252304.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  26. Positive feedback loop between Sox2 and Sox6 inhibits neuronal differentiation in the developing central nervous system.
    Authors: Lee, Kyung Eu, Seo, Jihae, Shin, Jiheon, Ji, Eun Hye, Roh, Jiwon, Kim, Joo Yeon, Sun, Woong, Muhr, Jonas, Lee, Sanghyuk, Kim, Jaesang
    Proc Natl Acad Sci U S A, 2014;111(7):2794-9.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  27. 3D imaging of Sox2 enhancer clusters in embryonic stem cells.
    Authors: Liu, Zhe, Legant, Wesley R, Chen, Bi-Chang, Li, Li, Grimm, Jonathan, Lavis, Luke D, Betzig, Eric, Tjian, Robert
    Elife, 2014;3(0):e04236.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
  28. MOF-associated complexes ensure stem cell identity and Xist repression.
    Authors: Chelmicki T, Dundar F, Turley M, Khanam T, Aktas T, Ramirez F, Gendrel A, Wright P, Videm P, Backofen R, Heard E, Manke T, Akhtar A
    Elife, 2014;3(0):e02024.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
  29. Role of SOX2 in the etiology of embryonal carcinoma, based on analysis of the NCCIT and NT2 cell lines.
    Authors: Eini, Ronak, Stoop, Hans, Gillis, Ad J M, Biermann, Katharin, Dorssers, Lambert, Looijenga, Leendert
    PLoS ONE, 2014;9(1):e83585.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC Paraffin-embedded
  30. Selective lentiviral gene delivery to CD133-expressing human glioblastoma stem cells.
    Authors: Bayin, N Sumru, Modrek, Aram S, Dietrich, August, Lebowitz, Jonathan, Abel, Tobias, Song, Hae-Ri, Schober, Markus, Zagzag, David, Buchholz, Christia, Chao, Moses V, Placantonakis, Dimitris
    PLoS ONE, 2014;9(12):e116114.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fluorescence
  31. In vitro generation of neuromesodermal progenitors reveals distinct roles for wnt signalling in the specification of spinal cord and paraxial mesoderm identity.
    Authors: Gouti, Mina, Tsakiridis, Anestis, Wymeersch, Filip J, Huang, Yali, Kleinjung, Jens, Wilson, Valerie, Briscoe, James
    PLoS Biol, 2014;12(8):e1001937.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  32. The germinal matrices in the developing dentate gyrus are composed of neuronal progenitors at distinct differentiation stages.
    Authors: Sugiyama T, Osumi N, Katsuyama Y
    Dev Dyn, 2013;242(12):1442-53.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: immunohistochemestry
  33. Transcription factors FOXG1 and Groucho/TLE promote glioblastoma growth.
    Authors: Verginelli F, Perin A, Dali R, Fung K, Lo R, Longatti P, Guiot M, Del Maestro R, Rossi S, di Porzio U, Stechishin O, Weiss S, Stifani S
    Nat Commun, 2013;4(0):2956.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  34. Sex determining region Y-box 2 (SOX2) amplification is an independent indicator of disease recurrence in sinonasal cancer.
    Authors: Schrock A, Goke F, Wagner P, Bode M, Franzen A, Braun M, Huss S, Agaimy A, Ihrler S, Menon R, Kirsten R, Kristiansen G, Bootz F, Lengerke C, Perner S
    PLoS ONE, 2013;8(3):e59201.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  35. SOX2 expression associates with stem cell state in human ovarian carcinoma.
    Authors: Bareiss, Petra M, Paczulla, Anna, Wang, Hui, Schairer, Rebekka, Wiehr, Stefan, Kohlhofer, Ursula, Rothfuss, Oliver C, Fischer, Anna, Perner, Sven, Staebler, Annette, Wallwiener, Diethelm, Fend, Falko, Fehm, Tanja, Pichler, Bernd, Kanz, Lothar, Quintanilla-Martinez, Leticia, Schulze-Osthoff, Klaus, Essmann, Frank, Lengerke, Claudia
    Cancer Res, 2013;73(17):5544-55.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Not specified
  36. Sox2 marks epithelial competence to generate teeth in mammals and reptiles.
    Authors: Juuri, Emma, Jussila, Maria, Seidel, Kerstin, Holmes, Scott, Wu, Ping, Richman, Joy, Heikinheimo, Kristiin, Chuong, Cheng-Mi, Arnold, Katrin, Hochedlinger, Konrad, Klein, Ophir, Michon, Frederic, Thesleff, Irma
    Development, 2013;140(7):1424-32.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  37. BEND6 is a nuclear antagonist of Notch signaling during self-renewal of neural stem cells.
    Authors: Dai Q, Andreu-Agullo C, Insolera R, Wong L, Shi S, Lai E
    Development, 2013;140(9):1892-902.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  38. Efficient generation of human iPSCs by a synthetic self-replicative RNA.
    Authors: Yoshioka N, Gros E, Li H, Kumar S, Deacon D, Maron C, Muotri A, Chi N, Fu X, Yu B, Dowdy S
    Cell Stem Cell, 2013;13(2):246-54.
    Species: Human
    Sample Type: Whole Cells
    Application: IF
  39. Genome-wide chromatin interactions of the Nanog locus in pluripotency, differentiation, and reprogramming.
    Authors: Apostolou E, Ferrari F, Walsh R, Bar-Nur O, Stadtfeld M, Cheloufi S, Stuart H, Polo J, Ohsumi T, Borowsky M, Kharchenko P, Park P, Hochedlinger K
    Cell Stem Cell, 2013;12(6):699-712.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: ChIP
  40. Sox2 cooperates with Chd7 to regulate genes that are mutated in human syndromes.
    Authors: Engelen E, Akinci U, Bryne JC
    Nat. Genet., 2011;43(6):607-11.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  41. Specific detection of OCT3/4 isoform A/B/B1 expression in solid (germ cell) tumours and cell lines: confirmation of OCT3/4 specificity for germ cell tumours.
    Authors: Rijlaarsdam MA, van Herk HA, Gillis AJ
    Br. J. Cancer, 2011;105(6):854-63.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  42. Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord.
    Authors: Belgacem YH, Borodinsky LN,
    Proc. Natl. Acad. Sci. U.S.A., 2011;108(11):4482-7.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: IHC
  43. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells.
    Authors: Klim JR, Li L, Wrighton PJ
    Nat. Methods, 2010;7(12):989-94.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  44. SIP1 mediates cell-fate decisions between neuroectoderm and mesendoderm in human pluripotent stem cells.
    Authors: Chng Z, Teo A, Pedersen RA, Vallier L
    Cell Stem Cell, 2010;6(1):59-70.
    Species: Human
    Sample Type: Cell Lysates
    Application: ChIP
  45. Activity-dependent expression of Lmx1b regulates specification of serotonergic neurons modulating swimming behavior.
    Authors: Demarque M, Spitzer NC
    Neuron, 2010;67(2):321-34.
    Species: Xenopus
    Sample Type: Whole Tissue
    Application: IHC Frozen
  46. Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens.
    Authors: Pollard SM, Yoshikawa K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire JA, Smith A, Dirks P
    Cell Stem Cell, 2009;4(6):568-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  47. Epigenetic-mediated dysfunction of the bone morphogenetic protein pathway inhibits differentiation of glioblastoma-initiating cells.
    Authors: Lee J, Son MJ, Woolard K, Donin NM, Li A, Cheng CH, Kotliarova S, Kotliarov Y, Walling J, Ahn S, Kim M, Totonchy M, Cusack T, Ene C, Ma H, Su Q, Zenklusen JC, Zhang W, Maric D, Fine HA
    Cancer Cell, 2008;13(1):69-80.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  48. Regulation of self-renewal and pluripotency by Sox2 in human embryonic stem cells.
    Authors: Fong H, Hohenstein KA, Donovan PJ
    Stem Cells, 2008;26(8):1931-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  49. Hyaluronan-CD44 interaction activates stem cell marker Nanog, Stat-3-mediated MDR1 gene expression, and ankyrin-regulated multidrug efflux in breast and ovarian tumor cells.
    Authors: Bourguignon LY, Peyrollier K, Xia W, Gilad E
    J. Biol. Chem., 2008;283(25):17635-51.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  50. Characterization of progenitor domains in the developing mouse thalamus.
    Authors: Vue TY, Aaker J, Taniguchi A
    J. Comp. Neurol., 2007;505(1):73-91.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  51. New cell lines from mouse epiblast share defining features with human embryonic stem cells.
    Authors: Tesar PJ, Chenoweth JG, Brook FA, Davies TJ, Evans EP, Mack DL, Gardner RL, McKay RD
    Nature, 2007;448(7150):196-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  52. Microglia instruct subventricular zone neurogenesis.
    Authors: Walton NM, Sutter BM, Laywell ED, Levkoff LH, Kearns SM, Marshall GP, Scheffler B, Steindler DA
    Glia, 2006;54(8):815-25.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
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Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 4 (Based on 1 review)

Have you used Human/Mouse/Rat SOX2 Antibody?

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Read what people are saying who have used Human/Mouse/Rat SOX2 Antibody.

We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Human/Mouse/Rat SOX2 Antibody AF2018
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Very Good
 IHC Human Anonymous 11/23/2017
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Immunohistochemistry Human/Mouse/Rat SOX2 Antibody AF2018
Immunohistochemistry: Human/Mouse/Rat SOX2 Antibody [AF2018]

Summary

ApplicationImmunohistochemistry
Sample TestedEmbryonic stem cells differentiated into dopaminergic neurons
SpeciesHuman

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