Human HNF-4 alpha/NR2A1 Antibody

R&D Systems | Catalog # PP-K9218-00

Isoforms 1-6
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # K9218
View all HNF-4 alpha/NR2A1 Primary Antibodies »

Product Specifications

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human HNF-4 alpha/NR2A1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using PP-K9218-00 in the following applications:

Formulation, Preparation, and Storage

Formulation

A liquid formulation in physiologic saline with 0.1% NaN3.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: HNF-4 alpha/NR2A1

Hepatocyte Nuclear Factor 4 alpha (HNF4 alpha; NR2A1) is a member of the orphan nuclear receptor family. HNF4 alpha is expressed in the liver, kidney, intestine and pancreas. Mutation of HNF4 alpha in humans has been associated with maturity-onset diabetes of the young type 1 (MODY1). HNF4 binds to DNA as an exclusive homodimer. The HNF4 alpha gene is alternatively spliced and may generate up to nine different isoforms, HNF4 alpha 1 through HNF4 alpha 9. The expression of HNF-4 gamma (NR2A2) is observed in the pancreas, kidney, small intestine, and testes with no expression detected in the liver. The level of HNF-4 gamma is less 10% than that of HNF-4 alpha.

Long Name

Hepatocyte Nuclear Factor-4, alpha

Alternate Names

HNF4 alpha, HNF4A, MODY1, NR2A1, TCF14

Entrez Gene IDs

3172 (Human)

Gene Symbol

HNF4A

Additional HNF-4 alpha/NR2A1 Products

Product Documents for Human HNF-4 alpha/NR2A1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human HNF-4 alpha/NR2A1 Antibody

For research use only

Citations for Human HNF-4 alpha/NR2A1 Antibody

Customer Reviews for Human HNF-4 alpha/NR2A1 Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Human HNF-4 alpha/NR2A1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Pancreas tissue
    Species: Human
    Verified Customer | Posted 11/22/2022
    Human HNF-4 alpha/NR2A1 Antibody PP-K9218-00
  • Human HNF-4 alpha/NR2A1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Hepatocytes
    Species: Human
    Verified Customer | Posted 12/17/2021
    Human HNF-4 alpha/NR2A1 Antibody PP-K9218-00

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Protocols

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FAQs

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Associated Pathways

TGF-beta Signaling Pathways TGF-beta Signaling Pathway Thumbnail