Choline Acetyltransferase/ChAT Antibody (CL3173)

Novus Biologicals | Catalog # NBP3-52467

Recombinant Monoclonal Antibody
Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # CL3173
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P28329, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (CL3173)

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of human placenta shows strong cytoplasmic positivity in a subset of cells in chorionic villi.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of mouse basal forebrain shows strong positivity in acetylcholine neurons in the caudate putamen.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of mouse brain shows strong positivity in acetylcholine neurons in the basal forebrain.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of rat brain shows strong positivity in acetylcholine neurons in the basal forebrain.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of rat brain shows strong positivity in acetylcholine neurons in the medial septum.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP3-52467]

Staining of SK-MEL-30 cells showing specific staining in the cytosol in green. Microtubule and nuclear probes are visualized in red and blue, respectively (where available).

Applications for Choline Acetyltransferase/ChAT Antibody (CL3173)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Preservative

0.02% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody (CL3173)

Certificate of Analysis

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (CL3173)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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