Choline Acetyltransferase/ChAT Antibody

Novus Biologicals | Catalog # NB110-89724

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry Free-Floating, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Choline Acetyltransferase/ChAT Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse Choline Acetyltransferase/ChAT conjugated to blue carrier protein was used as the antigen.

Marker

Cholinergic Neuronal Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Western Blot: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - Effect of denaturing temperature on Western blot on brain lysates using Rabbit antibody to ChAT.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IF on 1 day- old mouse cortical cell culture using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. DAPI counter stained.
Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord (free floating sections) at 1:1000 dilution using Rabbit antibody to c-terminal region of ChAT (choline O-acetyltransferase): whole serum (NB110-89724).
Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry Free-Floating: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on rat spinal cord using Rabbit antibody to rat, mouse ChAT (choline O-acetyltransferase): whole serum at 1:2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NB110-89724] - IHC on paraffin sections of rat DRG using Rabbit antibody to ChAT.

Applications for Choline Acetyltransferase/ChAT Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:2000

Immunohistochemistry

1:500

Immunohistochemistry Free-Floating

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500
Application Notes
This antibody is currently not validating in Immunohistochemistry-Frozen.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Choline Acetyltransferase/ChAT

Cholinergic systems are implicated in numerous neurologic functions. Alteration in some cholinergic neurons may account for the disturbances of Alzheimer disease. The protein encoded by this gene synthesizes the neurotransmitter acetylcholine. Alternative splice variants have been found that contain alternative 5' untranslated exons. Three of the four described splice variants encode identical 69 kDa proteins while one variant encodes both the 69 kDa and a larger 82 kDa protein. Choline acetylase catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. Defects in CHAT are the cause of familial infantile myasthenia gravis 2 (FIMG2); also known as CMS-EA. FIMG2 patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Inheritance is autosomal recessive.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

12647 (Mouse); 290567 (Rat)

Gene Symbol

CHAT

UniProt

P32738 (Rat)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Choline Acetyltransferase/ChAT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Choline Acetyltransferase/ChAT Antibody

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Choline Acetyltransferase/ChAT Antibody

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  • Q: I need Choline Acetyltransferase antibody - neuronal marker for the rat brain and only for immunohistochemistry frozen sections staining. In your catalog I see you have NB110-89724, NBP1-46764 and NB100-64603. Which one of it is suitable for our needs?

    A:

    Regarding your question, all three of those antibodies are suitable for rat frozen sections and you can choose any of the three; however, the immunogen for NB100-64603 is choline acetyltransferase purified from rat brain. Please note that the working dilution we recommend for this antibody is 1:100 to 1:250. Here is our general protocol for IHC-fr for your attention.

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