Clathrin interactor 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85570

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Clathrin interactor 1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADFGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Clathrin interactor 1 Antibody - BSA Free

Western Blot: Clathrin interactor 1 Antibody [NBP1-85570]

Western Blot: Clathrin interactor 1 Antibody [NBP1-85570]

Western Blot: Clathrin interactor 1 Antibody [NBP1-85570] - Analysis using Anti-CLINT1 antibody NBP1-85570 (A) shows similar pattern to independent antibody NBP2-49239 (B).
Immunocytochemistry/ Immunofluorescence: Clathrin interactor 1 Antibody [NBP1-85570]

Immunocytochemistry/ Immunofluorescence: Clathrin interactor 1 Antibody [NBP1-85570]

Immunocytochemistry/Immunofluorescence: Clathrin interactor 1 Antibody [NBP1-85570] - Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles. Antibody staining is shown in green.
Clathrin interactor 1 Antibody

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] - Staining of human lymph node shows strong cytoplasmic positivity in non-germinal center cells.
Clathrin interactor 1 Antibody

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -Staining of human kidney shows strong cytoplasmic/ membranous positivity in cells in tubules.
Clathrin interactor 1 Antibody

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Clathrin interactor 1 Antibody

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] -

Immunohistochemistry-Paraffin: Clathrin interactor 1 Antibody [NBP1-85570] - Staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Clathrin interactor 1 Antibody - BSA Free Western Blot: Clathrin interactor 1 Antibody - BSA Free [NBP1-85570]

Western Blot: Clathrin interactor 1 Antibody - BSA Free [NBP1-85570]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for Clathrin interactor 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Clathrin interactor 1

Epsin R is enriched in clathrin-coated vesicles and associates with clathrin and AP1 (adaptor protein complex 1). EpsinR plays a role in transport of vesicles in the endosomal/trans-Golgi network and at the synapses of neurons. The EpsinR gene has been associated with the genetic susceptibility to schizophrenia. Alternative names for EpsinR include Epsin-4, Epsin-related protein, enthoprotin, clathrin interactor 1, Clint, CLINT1, ENTH, EPN4, EPNR, and KIAA0171.

Alternate Names

clathrin interacting protein localized in the trans-Golgi region, clathrin interactor 1, Clathrin-interacting protein localized in the trans-Golgi region, CLINT, ENTHClint, enthoprotin, EPN4Enthoprotin, EPNRepsin 4, Epsin-4, EpsinR, Epsin-related protein, FLJ46753, KIAA0171epsinR

Gene Symbol

CLINT1

Additional Clathrin interactor 1 Products

Product Documents for Clathrin interactor 1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Clathrin interactor 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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