Key Product Details

Species Reactivity

Bacteria

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

DyLight 405 (Excitation = 400 nm, Emission = 420 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PCG4.1
View all Toxin A/TcdA Primary Antibodies »

Product Specifications

Immunogen

Full length protein A (C. difficile).

Reactivity Notes

Hamster reactivity reported in scientific literature (PMID: 27647869). Works with stool samples.

Localization

Secreted

Specificity

C. difficile Toxin A. Does not cross react with C. difficile Toxin B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Clostridium Difficile Toxin A Antibody (PCG4.1) [DyLight 405]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Toxin A/TcdA

Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhoea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.

Alternate Names

ToxA, Toxin A

Additional Toxin A/TcdA Products

Product Documents for Clostridium Difficile Toxin A Antibody (PCG4.1) [DyLight 405]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Clostridium Difficile Toxin A Antibody (PCG4.1) [DyLight 405]

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Clostridium Difficile Toxin A Antibody (PCG4.1) [DyLight 405]

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  • Q: I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?

    A: I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.

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