Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Bacteria

Cited:

Human, Bacteria, C. difficile, Hamster

Applications

Validated:

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # PCG4.1

Format

BSA Free
View all Toxin A/TcdA Primary Antibodies »

Product Specifications

Immunogen

Full length protein A (C. difficile).

Localization

Secreted

Specificity

C. difficile Toxin A. Does not cross react with C. difficile Toxin B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Clostridium-Difficile-Toxin-A-Antibody-PCG4-1-Immunocytochemistry-Immunofluorescence-NB600-1066-img0007.jpg
Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Clostridium-Difficile-Toxin-A-Antibody-PCG4-1-Immunocytochemistry-Immunofluorescence-NB600-1066-img0001.jpg
Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066]

Clostridium-Difficile-Toxin-A-Antibody-PCG4-1-Immunocytochemistry-Immunofluorescence-NB600-1066-img0006.jpg
Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] -

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] - PACSIN2 is required for TcdA uptake & toxin-induced rounding in wildtype MEF cells. (C), (D) & (E). Depletion of PACSIN2 reduces TcdA uptake in MEF cells. Wildtype MEF cells expressing luciferase (luc) or PACSIN2 siRNA incubated with 50 nM TcdA-546 at 10°C for 45 min. Cells allowed to warm up to 37°C for 2 min & then washed to remove unbound toxins & incubated with fresh media prewarmed to 37°C. Bound toxins allowed to internalize for 9 min at 37°C. Cells then fixed, stained for PACSIN2 & imaged by confocal microscopy. PACSIN2 & TcdA-546 staining from each condition shown in (C). Scale bars, 10 μm. The images shown representative of multiple fields imaged from two independent experiments. (D) Comparison of mean fluorescence intensities of PACSIN2 between luc & PACSIN2 siRNA transfected cells. Data represent mean & SD of 101 individual cells. Student’s t test ***p<0.0001. (E) Comparison of mean fluorescence intensities of TcdA-546 between luc & PACSIN2 siRNA transfected cells. Data represent mean & SD of 101 individual cells. Student’s t test ***p<0.0001. Cells chosen at random for intensity analyses. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006070), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] -

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] - TcdA colocalizes with PACSIN2 in wildtype MEF cells.(A) Wildtype MEFs on glass coverslips were allowed to bind 50 nM TcdA-546 for 45 min at 10°C, & cells were shifted to 37°C to allow internalization of toxin for 3 min. Cells were fixed, stained for PACSIN2, & analyzed by confocal microscopy. Merged images show PACSIN2 in red, toxin in green, & colocalization in yellow. Scale bars, 10 μm. The images shown are representative of multiple fields imaged from two independent experiments. (B) Immunofluorescence assays were performed as described in (A), but cells were stained for cav1 in addition to PACSIN2. Merged images show PACSIN2 in red, toxin in green, & cav1 in blue. Yellow puncta in merged images denote TcdA- & PACSIN2-positive structures. Pink punta denote caveolae-associated PACSIN2. Scale bars, 10 μm. (C) Pearson’s correlation coefficient to assess the extent of colocalization between PACSIN2, cav1 & TcdA-546 after 3 min toxin uptake. Data represent mean & SD of 31 individual cells chosen at random. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006070), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.
Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] -

Immunocytochemistry/ Immunofluorescence: Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free [NB600-1066] - TcdA colocalizes with PACSIN2 in wildtype MEF cells.(A) Wildtype MEFs on glass coverslips were allowed to bind 50 nM TcdA-546 for 45 min at 10°C, & cells were shifted to 37°C to allow internalization of toxin for 3 min. Cells were fixed, stained for PACSIN2, & analyzed by confocal microscopy. Merged images show PACSIN2 in red, toxin in green, & colocalization in yellow. Scale bars, 10 μm. The images shown are representative of multiple fields imaged from two independent experiments. (B) Immunofluorescence assays were performed as described in (A), but cells were stained for cav1 in addition to PACSIN2. Merged images show PACSIN2 in red, toxin in green, & cav1 in blue. Yellow puncta in merged images denote TcdA- & PACSIN2-positive structures. Pink punta denote caveolae-associated PACSIN2. Scale bars, 10 μm. (C) Pearson’s correlation coefficient to assess the extent of colocalization between PACSIN2, cav1 & TcdA-546 after 3 min toxin uptake. Data represent mean & SD of 31 individual cells chosen at random. Image collected & cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.ppat.1006070), licensed under a CC0-1.0 license. Not internally tested by Novus Biologicals.

Applications for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
WB reactivity reported in (PMID: 28346491). Works with stool samples.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM Sodium Phosphate (pH 7.4) and 0.15M NaCl

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.16 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Toxin A/TcdA

Clostridium difficile, or C. difficile, a gram positive anaerobic bacterium, is the major causative agent of colitis and diarrhoea that can occur following antibiotic intake. C. difficile infection is one of the most common hospital acquired infections. When C. difficile colonize the gut, they release two potent toxins, toxin A and toxin B, which bind to certain receptors in the lining of the colon and ultimately cause diarrhoea and inflammation of the large intestine.

Alternate Names

ToxA, Toxin A

Additional Toxin A/TcdA Products

Product Documents for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glycobiology-related Enzymes

Citations for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Clostridium Difficile Toxin A Antibody (PCG4.1) - BSA Free

Showing  1 - 3 of 3 FAQs Showing All

  • Q: I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?

    A: I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.

  • Q: Two questions: 1. Do you have any available data regarding the titer of NB600-1066, Anti C. difficile toxin A mAB PGC4 in ELISA? 2. I see that you sell other C. difficile toxin A and B antibodies. Is there any available ELISA data on those antibodies? And are they free from other nonspecific antibodies? Or are all of your mABs purified from ascites?

    A: 1. We do not have an in-house ELISA titer, but I have two publications that have used NB600-1066 in ELISA applications: PMID 21330434 and PMID 22851750. 2. For our other Clostridium Difficile products, the ELISA testing data came from either publications or from the clone inventors. We unfortunately do not have any in-house ELISA data for these products. Our mABs can be purified from either tissue culture supernatant or ascites. We try to include the source on our datasheet, but some of them are missing this information. If you have a specific product, we can always look this information up for you. Since our mABs are either sold unpurified or protein A/G purified, there is a possibility for some non-specific antibodies to be present. We unfortunately do not test our products for the presence of other antibodies.

  • Q: Was NB-600-1066 Clostridium Difficile Toxin A antibody PCG4.1 purified from ascites or tissue culture supernatant? We are in search of an ELISA grade anti- Tcd A antibody that does not contain other antibodies.

    A: Our Clostridium Difficile Toxin A Antibody catalog number NB600-1066 was protein A purified from mouse ascites.

  • Q: I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?

    A: I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.

  • Q: Two questions: 1. Do you have any available data regarding the titer of NB600-1066, Anti C. difficile toxin A mAB PGC4 in ELISA? 2. I see that you sell other C. difficile toxin A and B antibodies. Is there any available ELISA data on those antibodies? And are they free from other nonspecific antibodies? Or are all of your mABs purified from ascites?

    A: 1. We do not have an in-house ELISA titer, but I have two publications that have used NB600-1066 in ELISA applications: PMID 21330434 and PMID 22851750. 2. For our other Clostridium Difficile products, the ELISA testing data came from either publications or from the clone inventors. We unfortunately do not have any in-house ELISA data for these products. Our mABs can be purified from either tissue culture supernatant or ascites. We try to include the source on our datasheet, but some of them are missing this information. If you have a specific product, we can always look this information up for you. Since our mABs are either sold unpurified or protein A/G purified, there is a possibility for some non-specific antibodies to be present. We unfortunately do not test our products for the presence of other antibodies.

  • Q: Was NB-600-1066 Clostridium Difficile Toxin A antibody PCG4.1 purified from ascites or tissue culture supernatant? We are in search of an ELISA grade anti- Tcd A antibody that does not contain other antibodies.

    A: Our Clostridium Difficile Toxin A Antibody catalog number NB600-1066 was protein A purified from mouse ascites.

  • Q: I am seeking monoclonal antibodies specific for the C Terminal binding regions of clostridum difficile toxins A and B (aa1852-2366)?

    A: I would recommend catalog # NB100-62473. Although it is a polyclonal, it is the only one that we know binds to the C-Terminal portion of the protein. The other antibodies we have against both targets are synthesized from the full length sequence. We do not routinely epitope map our antibodies so we cannot be certain where they are binding. This polyclonal is synthesized against a small 10 amino acid region of the protein so it should be highly specific to that location.

  • Q: Two questions: 1. Do you have any available data regarding the titer of NB600-1066, Anti C. difficile toxin A mAB PGC4 in ELISA? 2. I see that you sell other C. difficile toxin A and B antibodies. Is there any available ELISA data on those antibodies? And are they free from other nonspecific antibodies? Or are all of your mABs purified from ascites?

    A: 1. We do not have an in-house ELISA titer, but I have two publications that have used NB600-1066 in ELISA applications: PMID 21330434 and PMID 22851750. 2. For our other Clostridium Difficile products, the ELISA testing data came from either publications or from the clone inventors. We unfortunately do not have any in-house ELISA data for these products. Our mABs can be purified from either tissue culture supernatant or ascites. We try to include the source on our datasheet, but some of them are missing this information. If you have a specific product, we can always look this information up for you. Since our mABs are either sold unpurified or protein A/G purified, there is a possibility for some non-specific antibodies to be present. We unfortunately do not test our products for the presence of other antibodies.

  • Q: Was NB-600-1066 Clostridium Difficile Toxin A antibody PCG4.1 purified from ascites or tissue culture supernatant? We are in search of an ELISA grade anti- Tcd A antibody that does not contain other antibodies.

    A: Our Clostridium Difficile Toxin A Antibody catalog number NB600-1066 was protein A purified from mouse ascites.

Showing  1 - 3 of 3 FAQs Showing All
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