Cofilin Antibody (567) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-42828

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (99%), Porcine (99%), Rhesus Macaque (99%), Sheep (99%). Backed by our 100% Guarantee.

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 567

Format

Azide and BSA Free
View all CFL1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin Antibody (567) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828]

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828]

Immunohistochemistry-Paraffin: Cofilin Antibody (567) [NBP2-42828] - HBL435 xenograft. Cofilin 1 antibody [567] dilution: 1:200. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Knockout Validated: Cofilin Antibody (567) [NBP2-42828]

Western Blot: Cofilin Antibody (567) [NBP2-42828]

Western Blot: Cofilin Antibody (567) [NBP2-42828] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567]. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Cofilin Antibody (567)

Western Blot: Cofilin Antibody (567) [NBP2-42828] -

Western Blot: Cofilin Antibody (567) [NBP2-42828] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Cofilin Antibody (567) - Azide and BSA Free

Western Blot: Cofilin Antibody (567) [NBP2-42828] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Cofilin Antibody (567) - Azide and BSA Free

Western Blot: Cofilin Antibody (567) [NBP2-42828] -

Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Cofilin Antibody (567) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody (567) [NBP2-42828] -

Cofilin 1 antibody [GT567] detects Cofilin 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 10 min.
Green: Cofilin 1 protein stained by Cofilin 1 antibody [GT567] (NBP2-42828) diluted at 1:300.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for Cofilin Antibody (567) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CFL1

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM]

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Gene Symbol

CFL1

Additional CFL1 Products

Product Documents for Cofilin Antibody (567) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cofilin Antibody (567) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Cofilin Antibody (567) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review Cofilin Antibody (567) - Azide and BSA Free and earn rewards!

Have you used Cofilin Antibody (567) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies