Collagen VII Antibody (LH7.2) - BSA Free

Novus Biologicals | Catalog # NB100-2728

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LH7.2

Format

BSA Free
View all Collagen VII Primary Antibodies »

Product Specifications

Immunogen

Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.

Epitope

The epitope is on the NC1 domain.

Localization

Secreted, extracellular space, extracellular matrix, basement membrane.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Collagen VII Antibody (LH7.2) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:10 - 1:500

Western Blot

1:100 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen VII

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Alternate Names

Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen

Entrez Gene IDs

1294 (Human)

Gene Symbol

COL7A1

Additional Collagen VII Products

Product Documents for Collagen VII Antibody (LH7.2) - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen VII Antibody (LH7.2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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