Connexin 26/GJB2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-41304

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat, Porcine

Cited:

Rat

Predicted:

Mouse (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Antibody was raised against a 16 amino acid peptide near the center of human GJB2. The immunogen is located within amino acids 80 - 130 of GJB2.

Reactivity Notes

Predicted species reactivity based on immunogen sequence: Sheep: (88%), Bovine: (88%), Chicken: (82%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Connexin 26/GJB2 Antibody - BSA Free

Western Blot: Connexin 26/GJB2 AntibodyBSA Free [NBP2-41304]

Western Blot: Connexin 26/GJB2 AntibodyBSA Free [NBP2-41304]

Connexin-26-GJB2-Antibody-Western-Blot-NBP2-41304-img0005.jpg
Immunocytochemistry/ Immunofluorescence: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Immunocytochemistry/ Immunofluorescence: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Connexin-26-GJB2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-41304-img0002.jpg
Immunohistochemistry: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Immunohistochemistry: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0004.jpg
Western Blot: Connexin 26/GJB2 AntibodyBSA Free [NBP2-41304]

Western Blot: Connexin 26/GJB2 AntibodyBSA Free [NBP2-41304]

Western Blot: Connexin 26/GJB2 Antibody [NBP2-41304] - Analysis of Connexin 26/GJB2 in human colon tissue lysate with Connexin 26/GJB2 antibody at (A) 1 and (B) 2 ug/ml.
Immunohistochemistry: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Immunohistochemistry: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304]

Connexin-26-GJB2-Antibody-Immunohistochemistry-NBP2-41304-img0003.jpg
Connexin 26/GJB2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Connexin 26/GJB2 Antibody - BSA Free [NBP2-41304] -

Cx26 distribution at level of the cardiomyocyte cytoplasm. (a–c) Confocal laser scanning microscopy: representative images of rat and pig heart sections. Double immunofluorescence analysis for Cx26 (red) and Cx43 (green) show a distinct cell localization of the two Cxs. (a) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal rat heart section treated with R alpha Cx26-cl and M alpha Cx43. Scale bar: 20 um. (b) Blow up of the square shows section view after clipping of some planes. Arrows point out the clusters of Cx26 and arrowheads intercalated discs where Cx43 is mainly localized. Scale bar: 10 um. (c) Three dimensional picture of the maximum intensity projection of the raw images of longitudinal pig heart section treated with R alpha Cx26-Ct and M alpha Cx43. Scale bar: 50 um. (d–f) Immuno-electron microscopy: representative pictures of immunocytochemistry for Cx26 in rat ventricle samples. Scale bars: 200 nm. (d) Cx26 immune-gold particles (arrows) are localized at level of myofibrils (mf) and cytoplasmic vesicles (cv) but not at the intercalated discs (arrowheads). (e) Cx26 gold particles (arrows) label mithocondria (M) and myofibrils (mf). (f) Negative control does not show Cx26 immunolabeling. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30228305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Connexin 26/GJB2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1 - 2 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33171391)

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Connexin 26/GJB2

Connexin-26 / GJB2 is a gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Each connexin is composed of a hexamer of connexin proteins. Connexins are a multi-gene family of highly related proteins. At least a dozen distinct connexin genes have been identified and many are expressed in a tissue-specific manner. Two distinct lineages, class I (beta) and class II (alpha), have been identified in mammals. Connexin-26 belongs to the class 1 (beta) group of connexins. Mutations in Connexin-26 are associated with genetically derived hearing impairments, including autosomal recessive non syndromic deafness.

Alternate Names

CX26, DFNA3, DFNB1, GJB2, HID, KID, NSRD1, PPK

Gene Symbol

GJB2

Additional Connexin 26/GJB2 Products

Product Documents for Connexin 26/GJB2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Connexin 26/GJB2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Citations for Connexin 26/GJB2 Antibody - BSA Free

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Protocols

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