CPSF6 Antibody

Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596]

Immunohistochemistry-Paraffin: CPSF6 Antibody [NB100-61596] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-CPSF68 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Simple Western: CPSF6 Antibody [NB100-61596]

Simple Western: CPSF6 Antibody [NB100-61596] - Simple Western lane view shows a specific band for CPSF6 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunoprecipitation: CPSF6 Antibody [NB100-61596]

Immunoprecipitation: CPSF6 Antibody [NB100-61596] - Detection of human CPSF68 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF68 antibody NB100-61596 (lot NB100-61596-2) used for IP at 3 ug per reaction. CPSF68 was also immunoprecipitated by a previous lot of this antibody (lot NB100-61596-1) and another rabbit anti-CPSF68 antibody. For blotting immunoprecipitated CPSF68, NB100-61596 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NB100-61596] -

Immunocytochemistry/ Immunofluorescence: CPSF6 Antibody [NB100-61596] - Depletion of TNPO3 does not change the localization of CPSF6. (A) TNPO3 K.D. & shRNA control HeLa cells were fixed & stained using specific antibodies against CPSF6 (red), ASF/SF2 (red) & TNPO3 (green), as described in Methods. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 1. (B) TNPO3 K.D. & shRNA control HeLa cells were transiently transfected using constructs expressing FLAG-tagged CPSF6 or ASF/SF2. Twenty-four hours post-transfection cells were fixed & immunostained using anti-FLAG antibodies. The nuclear compartment was labeled using DAPI. Image quantification is shown in Additional file 2. Similar results were obtained in three independent experiments & a representative experiment is shown. Image collected & cropped by CiteAb from the following publication (https://retrovirology.biomedcentral.com/articles/10.1186/1742-4690-10-46), licensed under a CC-BY license. Not internally tested by Novus Biologicals.