Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Predicted:
Bovine (94%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
CRBN antibody was raised against a 16 amino acid peptide near the carboxy terminus of human CRBN. The immunogen is located within the last 50 amino acids of CRBN. Amino Acid Squence: PTIPDTEDEISPDKVI
Specificity
At least two isoforms of CRBN are known to exist; this antibody will detect both isoforms.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for CRBN Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] -
Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079] - Validation of CRBN in HeLa Cells. Immunofluorescent analysis of methanol-fixed HeLa cells labeling CRBN with at 10 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and Hoechst staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.![Western Blot: CRBN AntibodyBSA Free [NBP2-82079]](https://resources.rndsystems.com/images/products/CRBN-Antibody-Western-Blot-NBP2-82079-img0001.jpg "Western Blot: CRBN AntibodyBSA Free [NBP2-82079]")
Western Blot: CRBN AntibodyBSA Free [NBP2-82079]
Western Blot: CRBN Antibody [NBP2-82079] - Analysis of CRBN in human testis tissue lysate with CRBN antibody at (A) 0.5 and (B) 1 ug/ml.![Immunohistochemistry: CRBN Antibody - BSA Free [NBP2-82079]](https://resources.rndsystems.com/images/products/CRBN-Antibody-Immunohistochemistry-NBP2-82079-img0002.jpg "Immunohistochemistry: CRBN Antibody - BSA Free [NBP2-82079]")
Immunohistochemistry: CRBN Antibody - BSA Free [NBP2-82079]
Immunohistochemistry: CRBN Antibody [NBP2-82079] - Immunohistochemistry of CRBN in rat testis tissue with CRBN antibody at 5 ug/mL.
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] -
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Human Cells. Loading: 15 ug of cell lysate Antibodies: CRBN, 1 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] -
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Mouse Tissues.Loading: 15 ug of mouse tissue lysate Antibodies: CRBN, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] -
Western Blot: CRBN Antibody - BSA Free [NBP2-82079] - Validation in Rat Tissues. Loading: 15 ug of rat tissue lysate Antibodies: CRBN, 0.5 ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.![Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079]](https://resources.rndsystems.com/images/products/CRBN-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-82079-img0003.jpg "Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079]")
Immunocytochemistry/ Immunofluorescence: CRBN Antibody - BSA Free [NBP2-82079]
Immunocytochemistry/Immunofluorescence: CRBN Antibody [NBP2-82079] - Immunofluorescence of CRBN in rat testis tissue with CRBN antibody at 20 ug/mL.Applications for CRBN Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
20 ug/ml
Immunohistochemistry
5 ug/ml
Western Blot
1-2 ug/ml
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: CRBN
Long Name
Cereblon
Alternate Names
Cereblon, MRT2, MRT2A, Protein X 0001
Gene Symbol
CRBN
Additional CRBN Products
Product Documents for CRBN Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CRBN Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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