Creatinine Parameter Assay Kit

  (28 citations)     
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Product Details
Assay Procedure
Citations (28)
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  • Format
    96-well strip plate
  • Assay Length
    < 1 hour
  • Sample Type & Volume Required Per Well
    Urine (10 uL)
  • Sensitivity
    0.07 mg/dL
  • Assay Range
    0.3 - 20 mg/dL (Urine)
  • Specificity
    Measures Creatinine levels in urine
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    Interference observed with other substances.
Control Available
QC69 , Parameter Immunoassay Control Set 685 for Creatinine - Please Inquire
Product Summary
The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Urine
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 1.67 5.28 9.75 1.55 4.92 9.25
Standard Deviation 0.053 0.168 0.339 0.085 0.259 0.366
CV% 3.2 3.2 3.5 5.5 5.3 4

Recovery

The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 96 92-97
Linearity
To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
 Creatinine [Biotin]
Product Datasheets

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Preparation and Storage
  • Storage
    Storage conditions vary by component. See product insert for storage instructions.
Background: Creatinine
Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.
  • Alternate Names:
    Creatinine
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well.

  5. 100 µL Alkaline Picrate Solution
  6.   Add 100 µL of Alkaline Picrate Solution to each well.
  7.   Incubate at room temperature for 25-35 minutes. Read at 490 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
Filter your results:

Species
Sample Type
  1. Dysregulated heme oxygenase-1low M2-like macrophages augment lupus nephritis via Bach1 induced by type I interferons
    Authors: D Kishimoto, Y Kirino, M Tamura, M Takeno, Y Kunishita, K Takase-Min, H Nakano, I Kato, K Nagahama, R Yoshimi, K Igarashi, I Aoki, H Nakajima
    Arthritis Res. Ther., 2018;20(1):64.
    Species: Mouse
    Sample Type: Urine
  2. Uromodulin deficiency alters tubular injury and interstitial inflammation but not fibrosis in experimental obstructive nephropathy
    Authors: O Maydan, PG McDade, Y Liu, XR Wu, DG Matsell, AA Eddy
    Physiol Rep, 2018;6(6):e13654.
    Species: Mouse
    Sample Type: Urine
  3. Prostaglandin D2amplifies lupus disease through basophil accumulation in lymphoid organs
    Authors: C Pellefigue, B Dema, Y Lamri, F Saidoune, N Chavarot, C Lohéac, E Pacreau, M Dussiot, C Bidault, F Marquet, M Jablonski, JM Chemouny, F Jouan, A Dossier, MP Chauveheid, D Gobert, T Papo, H Karasuyama, K Sacré, E Daugas, N Charles
    Nat Commun, 2018;9(1):725.
    Species: Mouse
    Sample Type: Urine
  4. Urinary kallikrein 10 predicts the incurability of gastric cancer
    Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
    Oncotarget, 2017;8(17):29247-29257.
    Species: Human
    Sample Type: Urine
  5. Response of Npt2a knockout mice to dietary calcium and phosphorus
    Authors: Y Li, D Caballero, J Ponsetto, A Chen, C Zhu, J Guo, M Demay, H Jüppner, C Bergwitz
    PLoS ONE, 2017;12(4):e0176232.
    Species: Mouse
    Sample Type: Urine
  6. Ameliorating Effect of Gemigliptin on Renal Injury in Murine Adriamycin-Induced Nephropathy
    Authors: DR Kim, SY Lee, JS Kim, YG Kim, JY Moon, SH Lee, TW Lee, CG Ihm, KH Jeong
    Biomed Res Int, 2017;2017(0):7275109.
    Species: Mouse
    Sample Type: Urine
  7. Urinary biomarkers as indicator of chronic inflammation and endothelial dysfunction in obese adolescents
    Authors: R Singh, A Verma, S Aljabari, TL Vasylyeva
    BMC Obes, 2017;4(0):11.
    Species: Human
    Sample Type: Urine
  8. Assessment of complementary feeding of Canadian infants: effects on microbiome & oxidative stress, a randomized controlled trial
    Authors: W Qasem, MB Azad, Z Hossain, E Azad, S Jorgensen, S Castillo S, C Cai, E Khafipour, T Beta, LJ Roberts, J Friel
    BMC Pediatr, 2017;17(1):54.
    Species: Human
    Sample Type: Urine
  9. A 12-week randomized, double-blind, placebo-controlled multicenter study of choline-stabilized orthosilicic acid in patients with symptomatic knee osteoarthritis
    Authors: P Geusens, K Pavelka, J Rovensky, J Vanhoof, N Demeester, M Calomme, D Vanden Ber
    BMC Musculoskelet Disord, 2017;18(1):2.
    Species: Human
    Sample Type: Urine
  10. Immune Activation in the Female Genital Tract: Expression Profiles of Soluble Proteins in Women at High Risk for HIV Infection.
    Authors: Francis S, Hou Y, Baisley K, van de Wijgert J, Watson-Jones D, Ao T, Herrera C, Maganja K, Andreasen A, Kapiga S, Coulton G, Hayes R, Shattock R
    PLoS ONE, 2016;11(1):e0143109.
    Species: Human
    Sample Type: Urine
  11. Metabolomic screening using ESI-FT MS identifies potential radiation-responsive molecules in mouse urine
    Authors: Daisuke Iizuka
    J. Radiat. Res, 2016;0(0):.
    Species: Mouse
    Sample Type: Urine
  12. Fas/CD95 prevents autoimmunity independently of lipid raft localization and efficient apoptosis induction
    Nat Commun, 2016;7(0):13895.
    Species: Mouse
    Sample Type: Urine
  13. Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with Obstructive Sleep Apnea
    PLoS ONE, 2016;11(5):e0154503.
    Species: Human
    Sample Type: Urine
  14. A multiplex immunoassay for the non-invasive detection of bladder cancer.
    Authors: Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C
    J Transl Med, 2016;14(1):31.
    Species: Human
    Sample Type: Urine
  15. Urinary L-FABP predicts poor outcomes in critically ill patients with early acute kidney injury.
    Authors: Parr, Sharidan, Clark, Amanda J, Bian, Aihua, Shintani, Ayumi K, Wickersham, Nancy E, Ware, Lorraine, Ikizler, T Alp, Siew, Edward D
    Kidney Int, 2015;87(3):640-8.
    Species: Human
    Sample Type: Urine
  16. A multi-analyte assay for the non-invasive detection of bladder cancer.
    Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
    PLoS ONE, 2012;7(10):e47469.
    Species: Human
    Sample Type: Urine
  17. CCL18 in a multiplex urine-based assay for the detection of bladder cancer.
    Authors: Urquidi V, Kim J, Chang M, Dai Y, Rosser CJ, Goodison S
    PLoS ONE, 2012;7(5):e37797.
    Species: Human
    Sample Type: Urine
  18. Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
    Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
    Am. J. Pathol., 2011;179(3):1179-87.
    Species: Mouse
    Sample Type: Urine
  19. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury.
    Authors: Jeansson M, Gawlik A, Anderson G
    J. Clin. Invest., 2011;121(6):2278-89.
    Species: Mouse
    Sample Type: Urine
  20. Poly(ADP-ribose) polymerase (PARP) inhibition counteracts multiple manifestations of kidney disease in long-term streptozotocin-diabetic rat model.
    Authors: Shevalye H, Stavniichuk R, Xu W, Zhang J, Lupachyk S, Maksimchyk Y, Drel VR, Floyd EZ, Slusher B, Obrosova IG
    Biochem. Pharmacol., 2010;79(7):1007-14.
    Species: Rat
    Sample Type: Urine
  21. Basophils and the T helper 2 environment can promote the development of lupus nephritis.
    Authors: Charles N, Hardwick D, Daugas E
    Nat. Med., 2010;16(6):701-7.
    Species: Mouse
    Sample Type: Urine
  22. Impact of elevated dietary sodium intake on NAD(P)H oxidase and SOD in the cortex and medulla of the rat kidney.
    Authors: Johns EJ, O'Shaughnessy B, O'Neill S, Lane B, Healy V
    Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;299(1):R234-40.
    Species: Rat
    Sample Type: Urine
  23. Systemic autoimmunity and defective Fas ligand secretion in the absence of the Wiskott-Aldrich syndrome protein.
    Authors: Nikolov NP, Shimizu M, Cleland S
    Blood, 2010;116(5):740-7.
    Species: Mouse
    Sample Type: Urine
  24. A urinary biomarker profile for children with HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
    Kidney Int., 2009;76(2):207-14.
    Species: Human
    Sample Type: Serum
  25. Poly(Adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy.
    Authors: Drel VR, Xu W, Zhang J, Pavlov IA, Shevalye H, Slusher B, Obrosova IG
    Endocrinology, 2009;150(12):5273-83.
    Species: Rat
    Sample Type: Urine
  26. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
    Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
    Clin J Am Soc Nephrol, 2009;4(4):763-71.
    Species: Human
    Sample Type: Urine
  27. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation.
    Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K
    Nature, 2008;454(7204):656-60.
    Species: Mouse
    Sample Type: Urine
  28. Regulation of the sodium/sulfate co-transporter by farnesoid X receptor alpha.
    Authors: Lee H, Hubbert ML, Osborne TF, Woodford K, Zerangue N, Edwards PA
    J. Biol. Chem., 2007;282(30):21653-61.
    Species: Mouse
    Sample Type: Urine
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Parameter Immunoassay Control Set 685 for Creatinine

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