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Creatinine Parameter Assay Kit

R&D Systems | Catalog # KGE005

R&D Systems
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Key Product Details

Assay Length

1 hour

Sample Type & Volume Required Per Well

Urine (10 µL)

Sensitivity

0.07 mg/dL

Assay Range

0.31-20 mg/dL (Urine)
View all Creatinine ELISA Kits »

Product Summary for Creatinine Parameter Assay Kit

The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Multi-Species

Specificity

Measures Creatinine levels in urine

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. Cross-species reactivity observed with 1 or more species tested.

Interference

Interference observed with other substances.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 1.67 5.28 9.75 1.55 4.92 9.25
Standard Deviation 0.053 0.168 0.339 0.085 0.259 0.366
CV% 3.2 3.2 3.5 5.5 5.3 4.0

Recovery for Creatinine Parameter Assay Kit

The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 96 92-97

Linearity

To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.

Multi-species Creatinine ELISA Linearity

Scientific Data Images for Creatinine Parameter Assay Kit

Multi-species Creatinine ELISA Standard Curve

Multi-species Creatinine ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage conditions vary by component. See product insert for storage instructions.

Background: Creatinine

Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.

Additional Creatinine Products

Product Documents for Creatinine Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Creatinine Parameter Assay Kit

For research use only

Related Research Areas

Diabetes

Citations for Creatinine Parameter Assay Kit

Customer Reviews for Creatinine Parameter Assay Kit (5)

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  • Creatinine Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 02/03/2021
    Creatinine Parameter Assay Kit KGE005
  • Creatinine Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 04/05/2019
  • Creatinine Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 12/17/2018
    Creatinine Parameter Assay Kit KGE005
  • Creatinine Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Purified Standard
    Verified Customer | Posted 09/11/2018
    Creatinine Parameter Assay Kit KGE005
  • Creatinine Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Urine
    Verified Customer | Posted 08/16/2018
    Creatinine Parameter Assay Kit KGE005

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Protocols

View specific protocols for Creatinine Parameter Assay Kit (KGE005):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Standard, Control, or Sample
  3.   Add 50 µL of Standard, control, or sample to each well.
    4. 100 µL Alkaline Picrate Solution
  4.   Add 100 µL of Alkaline Picrate Solution to each well.
  5.   Incubate at room temperature for 25-35 minutes. Read at 490 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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