Creatinine Parameter Assay Kit

(24 citations)   
  • Format
    96-well strip plate
  • Assay Length
    < 1 hour
  • Sample Type & Volume Required Per Well
    Urine (10 uL)
  • Sensitivity
    0.07 mg/dL
  • Assay Range
    0.3 - 20 mg/dL (Urine)
  • Specificity
    Measures Creatinine levels in urine
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
  • Interference
    Interference observed with other substances.
Control Available
QC69, Parameter Immunoassay Control Set 685 for Creatinine - Please Inquire
Product Summary
The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean1.675.289.751.554.929.25
Standard Deviation0.0530.1680.3390.0850.2590.366
CV%3.23.23.55.55.34

Recovery

The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Urine (n=4) 96 92-97
Linearity
To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
Creatinine Parameter Assay Kit
Preparation and Storage
  • Storage
    Storage conditions vary by component. See product insert for storage instructions.
Background: Creatinine
Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.
    • Alternate Names
      Creatinine;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Standard, Control, or Sample
    4.   Add 50 µL of Standard, control, or sample to each well.

    5. 100 µL Alkaline Picrate Solution
    6.   Add 100 µL of Alkaline Picrate Solution to each well.
    7.   Incubate at room temperature for 25-35 minutes. Read at 490 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    24 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Urinary kallikrein 10 predicts the incurability of gastric cancer
      Authors: T Shimura, M Ebi, T Yamada, T Yamada, T Katano, Y Nojiri, H Iwasaki, S Nomura, N Hayashi, Y Mori, H Kataoka, MA Moses, T Joh
      Oncotarget, 2017;8(17):29247-29257.
      Species: Human
      Sample Type: Urine
    2. Response of Npt2a knockout mice to dietary calcium and phosphorus
      Authors: Y Li, D Caballero, J Ponsetto, A Chen, C Zhu, J Guo, M Demay, H Jüppner, C Bergwitz
      PLoS ONE, 2017;12(4):e0176232.
      Species: Mouse
      Sample Type: Urine
    3. Ameliorating Effect of Gemigliptin on Renal Injury in Murine Adriamycin-Induced Nephropathy
      Authors: DR Kim, SY Lee, JS Kim, YG Kim, JY Moon, SH Lee, TW Lee, CG Ihm, KH Jeong
      Biomed Res Int, 2017;2017(0):7275109.
      Species: Mouse
      Sample Type: Urine
    4. Urinary biomarkers as indicator of chronic inflammation and endothelial dysfunction in obese adolescents
      Authors: R Singh, A Verma, S Aljabari, TL Vasylyeva
      BMC Obes, 2017;4(0):11.
      Species: Human
      Sample Type: Urine
    5. Assessment of complementary feeding of Canadian infants: effects on microbiome & oxidative stress, a randomized controlled trial
      Authors: W Qasem, MB Azad, Z Hossain, E Azad, S Jorgensen, S Castillo S, C Cai, E Khafipour, T Beta, LJ Roberts, J Friel
      BMC Pediatr, 2017;17(1):54.
      Species: Human
      Sample Type: Urine
    6. A 12-week randomized, double-blind, placebo-controlled multicenter study of choline-stabilized orthosilicic acid in patients with symptomatic knee osteoarthritis
      Authors: P Geusens, K Pavelka, J Rovensky, J Vanhoof, N Demeester, M Calomme, D Vanden Ber
      BMC Musculoskelet Disord, 2017;18(1):2.
      Species: Human
      Sample Type: Urine
    7. Fas/CD95 prevents autoimmunity independently of lipid raft localization and efficient apoptosis induction
      Nat Commun, 2016;7(0):13895.
      Species: Mouse
      Sample Type: Urine
    8. Urinary Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Patients with Obstructive Sleep Apnea
      PLoS ONE, 2016;11(5):e0154503.
      Species: Human
      Sample Type: Urine
    9. Immune Activation in the Female Genital Tract: Expression Profiles of Soluble Proteins in Women at High Risk for HIV Infection.
      Authors: Francis S, Hou Y, Baisley K, van de Wijgert J, Watson-Jones D, Ao T, Herrera C, Maganja K, Andreasen A, Kapiga S, Coulton G, Hayes R, Shattock R
      PLoS ONE, 2016;11(1):e0143109.
      Species: Human
      Sample Type: Urine
    10. A multiplex immunoassay for the non-invasive detection of bladder cancer.
      Authors: Shimizu Y, Furuya H, Bryant Greenwood P, Chan O, Dai Y, Thornquist M, Goodison S, Rosser C
      J Transl Med, 2016;14(1):31.
      Species: Human
      Sample Type: Urine
    11. Urinary L-FABP predicts poor outcomes in critically ill patients with early acute kidney injury.
      Authors: Parr, Sharidan, Clark, Amanda J, Bian, Aihua, Shintani, Ayumi K, Wickersham, Nancy E, Ware, Lorraine, Ikizler, T Alp, Siew, Edward D
      Kidney Int, 2015;87(3):640-8.
      Species: Human
      Sample Type: Urine
    12. CCL18 in a multiplex urine-based assay for the detection of bladder cancer.
      Authors: Urquidi V, Kim J, Chang M, Dai Y, Rosser CJ, Goodison S
      PLoS ONE, 2012;7(5):e37797.
      Species: Human
      Sample Type: Urine
    13. A multi-analyte assay for the non-invasive detection of bladder cancer.
      Authors: Goodison, Steve, Chang, Myron, Dai, Yunfeng, Urquidi, Virginia, Rosser, Charles
      PLoS ONE, 2012;7(10):e47469.
      Species: Human
      Sample Type: Urine
    14. Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy.
      Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR
      Am. J. Pathol., 2011;179(3):1179-87.
      Species: Mouse
      Sample Type: Urine
    15. Angiopoietin-1 is essential in mouse vasculature during development and in response to injury.
      Authors: Jeansson M, Gawlik A, Anderson G
      J. Clin. Invest., 2011;121(6):2278-89.
      Species: Mouse
      Sample Type: Urine
    16. Poly(ADP-ribose) polymerase (PARP) inhibition counteracts multiple manifestations of kidney disease in long-term streptozotocin-diabetic rat model.
      Authors: Shevalye H, Stavniichuk R, Xu W, Zhang J, Lupachyk S, Maksimchyk Y, Drel VR, Floyd EZ, Slusher B, Obrosova IG
      Biochem. Pharmacol., 2010;79(7):1007-14.
      Species: Rat
      Sample Type: Urine
    17. Basophils and the T helper 2 environment can promote the development of lupus nephritis.
      Authors: Charles N, Hardwick D, Daugas E
      Nat. Med., 2010;16(6):701-7.
      Species: Mouse
      Sample Type: Urine
    18. Impact of elevated dietary sodium intake on NAD(P)H oxidase and SOD in the cortex and medulla of the rat kidney.
      Authors: Johns EJ, O'Shaughnessy B, O'Neill S, Lane B, Healy V
      Am. J. Physiol. Regul. Integr. Comp. Physiol., 2010;299(1):R234-40.
      Species: Rat
      Sample Type: Urine
    19. Systemic autoimmunity and defective Fas ligand secretion in the absence of the Wiskott-Aldrich syndrome protein.
      Authors: Nikolov NP, Shimizu M, Cleland S
      Blood, 2010;116(5):740-7.
      Species: Mouse
      Sample Type: Urine
    20. A urinary biomarker profile for children with HIV-associated renal diseases.
      Authors: Soler-Garcia AA, Rakhmanina NY, Mattison PC, Ray PE
      Kidney Int., 2009;76(2):207-14.
      Species: Human
      Sample Type: Serum
    21. Poly(Adenosine 5'-diphosphate-ribose) polymerase inhibition counteracts multiple manifestations of experimental type 1 diabetic nephropathy.
      Authors: Drel VR, Xu W, Zhang J, Pavlov IA, Shevalye H, Slusher B, Obrosova IG
      Endocrinology, 2009;150(12):5273-83.
      Species: Rat
      Sample Type: Urine
    22. Iron-related proteins: candidate urine biomarkers in childhood HIV-associated renal diseases.
      Authors: Soler-Garcia AA, Johnson D, Hathout Y, Ray PE
      Clin J Am Soc Nephrol, 2009;4(4):763-71.
      Species: Human
      Sample Type: Urine
    23. Blocking VEGFR-3 suppresses angiogenic sprouting and vascular network formation.
      Authors: Tammela T, Zarkada G, Wallgard E, Murtomaki A, Suchting S, Wirzenius M, Waltari M, Hellstrom M, Schomber T, Peltonen R, Freitas C, Duarte A, Isoniemi H, Laakkonen P, Christofori G, Yla-Herttuala S, Shibuya M, Pytowski B, Eichmann A, Betsholtz C, Alitalo K
      Nature, 2008;454(7204):656-60.
      Species: Mouse
      Sample Type: Urine
    24. Regulation of the sodium/sulfate co-transporter by farnesoid X receptor alpha.
      Authors: Lee H, Hubbert ML, Osborne TF, Woodford K, Zerangue N, Edwards PA
      J. Biol. Chem., 2007;282(30):21653-61.
      Species: Mouse
      Sample Type: Urine
    Expand to show all 24 Citations
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    Parameter Immunoassay Control Set 685 for Creatinine

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