CRHR1/CRF1 Antibody - BSA Free

Novus Biologicals | Catalog # NLS1778

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Predicted:

Primate (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

Synthetic 17 amino acid peptide from N-terminal extracellular domain of human CRHR1/CRF1.

Epitope

N-Terminus extracellular domain

Reactivity Notes

Summary of Expression: CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries. Predicted cross-reactivity based on sequence identity: Marmoset (94%), Rat (82%).

Localization

Secreted

Specificity

Human CRF1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for CRHR1/CRF1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody - BSA Free [NLS1778]

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody - BSA Free [NLS1778]

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1778] - Analysis of anti-CRF1 antibody with rat hypothalamus.
Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody - BSA Free [NLS1778]

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody - BSA Free [NLS1778]

Immunohistochemistry-Paraffin: CRHR1/CRF1 Antibody [NLS1778] - Analysis of anti-CRF1 antibody with human brain, neurons and glia at 13 ug/ml.

Applications for CRHR1/CRF1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

13 ug/ml

Immunohistochemistry-Paraffin

13 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID : 29082104). Knockdown validation (PMID: 31881185). Use in Western Blot reported in scientific literature (PMID:31881185)..

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: CRHR1

Corticotropin-releasing factor receptor 1 (CRFR1/CRHR1), a Corticotropin-Releasing Factor Receptor, is involved in mediating the stress response and anxiety-related behaviors. Mutations of the receptor reduce anxiety-related behaviors and may be involved in the pathogenesis of Cushing disease. Multiple isoforms of CRFR1 are produced by alternative splicing. CRFR1 has been reported to be expressed in the pituitary, adrenal gland, brain, colon, ovary, placenta, testis, white blood cells, and skin. ESTs have been isolated from human brain, eye, colon, and embryo libraries.

Long Name

Corticotropin Releasing Hormone Receptor 1

Alternate Names

CHR-R1, CRF1, CRFR, CRFR1

Entrez Gene IDs

1394 (Human); 58959 (Rat)

Gene Symbol

CRHR1

UniProt

P34998 (Human)

Additional CRHR1 Products

Product Documents for CRHR1/CRF1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CRHR1/CRF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CRHR1/CRF1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CRHR1/CRF1 Antibody - BSA Free

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  • Q: He wonders whether it is suitable for mouse frozen section by IF. Can you so kind to help him and me?

    A: Our CRF1 antibody with catalogue number NLS1778 has been validated for use with samples from mouse, however it has only currently been tested for immunohistochemistry with paraffin-embedded tissue, and for immunocytochemistry. It has not yet been tested for immunohistochemistry with frozen tissue. Please refer your customer to our Innovator's Reward if they wish to try using this antibody for IHC with their frozen mouse tissue samples.

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