CRM1 Antibody - BSA Free

Western Blot: CRM1 Antibody [NB100-79802]

Western Blot: CRM1 Antibody [NB100-79802] - A) Whole cell lysate from HeLa (5, 15 and 50 mcg), Ramos (50 mcg) and mouse NIH3T3 (50 mcg) cells. B) Whole cell lysate (1 mg/IP; 1/4 of reaction loaded/lane) from HeLa cells. NB100-79802 used at 0.1 mcg/ml for WB (A and B) and at 3 mcg/mg lysate for IP. CRM1 was also less efficiently immunoprecipitated by NB100-79811, which recognize upstream epitopes on CRM1.

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802]

CRM1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-79802-img0027.jpg

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802]

Immunohistochemistry-Paraffin: CRM1 Antibody [NB100-79802] - Human lung cancer. Antibody: Affinity purified rabbit anti-CRM1 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Simple Western: CRM1 Antibody [NB100-79802]

Simple Western: CRM1 Antibody [NB100-79802] - Simple Western lane view shows a specific band for CRM1 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/Immunofluorescence: CRM1 Antibody [NB100-79802] -

A – Fluorescence images of cells transfected with thepTurboGFP-N-PON2 plasmid and then stained with anti-CRM1 antibodies. B– Fluorescence images of cells transfected with plasmidsencoding different fragments of PON2 (1–27 a.a.; 1–83 a.a.;1–168 a.a.) or GFP alone as a control

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - Effect of TSA treatment on the subcellular localization of Crm1.Control ES or Nup98-HoxA9 expressing ES cells were cultured in the presence or absence of 50 nM TSA for 24 hr. Then, the cells were fixed & stained with antibodies against FLAG (M2) & Crm1. Merged images of FLAG (green) & Crm1 (red) are shown. Nuclei were stained with DAPI. Bar, 10 μm. DAPI, 4',6-diamidino-2-phenylindole; ES, embryonic stem; TSA, trichostatin A.DOI:http://dx.doi.org/10.7554/eLife.09540.020 Image collected & cropped by CiteAb from the following publication (https://elifesciences.org/articles/09540), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] -

Immunocytochemistry/ Immunofluorescence: CRM1 Antibody [NB100-79802] - A – Fluorescence images of cells transfected with thepTurboGFP-N-PON2 plasmid & then stained with anti-CRM1 antibodies. B– Fluorescence images of cells transfected with plasmidsencoding different fragments of PON2 (1–27 a.a.; 1–83 a.a.;1–168 a.a.) or GFP alone as a control Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30397533), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CRM1 Antibody - BSA Free [NB100-79802] -

CRM1 expression and activity increased during normal aging. (a) Primary human fibroblast from healthy individuals of varying ages or HGPS‐1 fibroblasts were analyzed by Western blotting using antibodies against CRM1, lamin A/C, and actin (control). CRM1 expression is shown (bottom panel; unpaired t test). (b) Localization of the NES‐containing proteins STAT3, Z0‐2, and B23 was evaluated in the indicated fibroblast cultures, treated with LMB or vehicle alone for 24 hr. Typical images are shown. Bar, 20 uM. (c) The n/c ratio of STAT3, Z0‐2, and B23 was calculated as peer Methods (n = 50 cells; Mann–Whitney U test) Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31305018), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: CRM1 Antibody - BSA Free [NB100-79802] -

(a‐b) Enhanced nuclear export activity due to CRM1 overexpression overcomes deficient Ran gradient in HeLa cells. Cells were double‐transfected to stably expressed Flag‐CRM1 or Flag alone, and a shRNA against NTF2 gene or a shRNA control. (a) Lysates from the transfected cells were analyzed by Western blotting using antibodies against CRM1, NTF2, and actin (control). Middle. Relative protein levels were assessed from three independent experiments (unpaired t test). Right. Distribution of STAT3 was analyzed in the indicated transfected cells. Bar, 20 uM. (b) Transfected cell lysates were analyzed by Western blotting with antibodies against lamin B1, H3K9me, and actin (control). Middle. Data correspond to 3 independent experiments (unpaired t test). Right. Distribution of H3K9me3 was analyzed in the indicated transfected cells. Bar, 20 uM. (c–e) Restoration of lamin B1 expression in HGPS cells (c) HGPS‐1 cells were transiently transfected to express GFP‐lamin B1 or GFP alone. Transfected cells were immunolabeled for lamin A/C (d) and H3K9m3 (e) to estimate the percentage of cells with aberrant nuclear morphology and heterochromatin loss, respectively. Bar, 10 uM Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31305018), licensed under a CC-BY license. Not internally tested by Novus Biologicals.