CROCC Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80820

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VKRLEKQNLEKDQVNKDLTEKLEALESLRLQEQAALETEDGEGLQQTLRDLAQAVLSDSESGVQLSGSERTADASNGS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CROCC Antibody - BSA Free

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining in human fallopian tube and liver tissues using NBP1-80820 antibody. Corresponding CROCC RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus, fallopian tube, liver and testis using Anti-CROCC antibody NBP1-80820 (A) shows similar protein distribution across tissues to independent antibody NBP1-80821 (B).
Western Blot: CROCC Antibody [NBP1-80820]

Western Blot: CROCC Antibody [NBP1-80820]

Western Blot: CROCC Antibody [NBP1-80820] - Analysis in human cell line SCLC-21H.
Immunocytochemistry/ Immunofluorescence: CROCC Antibody [NBP1-80820]

Immunocytochemistry/ Immunofluorescence: CROCC Antibody [NBP1-80820]

Immunocytochemistry/Immunofluorescence: CROCC Antibody [NBP1-80820] - Staining of human cell line U-2 OS shows localization to plasma membrane, centrosome & actin filaments. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human bronchus shows strong positivity in apical membrane in respiratory epithelial cells.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human Fallopian tube shows strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820]

Immunohistochemistry-Paraffin: CROCC Antibody [NBP1-80820] - Staining of human testis shows very weak cytoplasmic positivity in Leydig cells.
CROCC Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CROCC Antibody - BSA Free [NBP1-80820] -

Endogenous cNap1 bridges proximal centrioles at the nanoscale. (A–C) U-ExM of centrioles labelled with anti-acetylated tubulin antibody (pink) and cNap1 labelled with anti-cNap1 antibody (grey). The images show single Airyscan z-slices. Cartoons depict simplified centriole and cNap1 orientations. (D) ExM of centrioles labelled with anti-acetylated tubulin antibody (pink) and cNap1 labelled with anti-cNap1 antibody (grey) in a ciliated HPNE cell. The image shows a maximum intensity projection from 3D data. The cartoon depicts only the ciliary base region for simplicity. (E) U-ExM of rootlets stained with anti-rootletin antibody (green) and centrioles stained with anti-acetylated tubulin antibody (pink). (F) U-ExM of rootlets stained with anti-rootletin antibody (green) and centrioles stained with gamma-tubulin antibody (yellow). Single z-slices are shown. Panels (A), (C), (E), (F) show wild-type U2OS, (B) is endogenously tagged cNap1-mScarlet-I U2OS and (D) shows hTERT-HPNE cells. Across all panels, the scale is 200 nm, and each column represents a different cell. Data underlying this figure can be found in S2 Fig. U-ExM, ultra-expansion microscopy. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36282799), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
CROCC Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CROCC Antibody - BSA Free [NBP1-80820] -

Endogenous cNap1 bridges proximal centrioles at the nanoscale. (A–C) U-ExM of centrioles labelled with anti-acetylated tubulin antibody (pink) and cNap1 labelled with anti-cNap1 antibody (grey). The images show single Airyscan z-slices. Cartoons depict simplified centriole and cNap1 orientations. (D) ExM of centrioles labelled with anti-acetylated tubulin antibody (pink) and cNap1 labelled with anti-cNap1 antibody (grey) in a ciliated HPNE cell. The image shows a maximum intensity projection from 3D data. The cartoon depicts only the ciliary base region for simplicity. (E) U-ExM of rootlets stained with anti-rootletin antibody (green) and centrioles stained with anti-acetylated tubulin antibody (pink). (F) U-ExM of rootlets stained with anti-rootletin antibody (green) and centrioles stained with gamma-tubulin antibody (yellow). Single z-slices are shown. Panels (A), (C), (E), (F) show wild-type U2OS, (B) is endogenously tagged cNap1-mScarlet-I U2OS and (D) shows hTERT-HPNE cells. Across all panels, the scale is 200 nm, and each column represents a different cell. Data underlying this figure can be found in S2 Fig. U-ExM, ultra-expansion microscopy. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36282799), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for CROCC Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CROCC

Long Name

Ciliary Rootlet Coiled-Coil, Rootletin

Alternate Names

CROCC1, KIAA0445, ROLT, TAX1BP2

Entrez Gene IDs

9696 (Human)

Gene Symbol

CROCC

Additional CROCC Products

Product Documents for CROCC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CROCC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CROCC Antibody - BSA Free

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Protocols

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