CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43648

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Predicted:

Bovine (95%), Porcine (95%), Xenopus (91%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1761

Format

Azide and BSA Free
View all CS Citrate Synthase Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Citrate synthetase. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (91%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse kidney. Citrate synthetase antibody [1761] dilution: 1:200.
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract E. 30 ug A375 whole cell lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 30 ug zebrafish brain lysate/extract 10 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000
Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of A. 50 ug mouse brain lysate/extract 12 % SDS-PAGE Citrate synthetase antibody [1761] dilution: 1:1000.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of H1299 xenograft. Citrate synthetase antibody dilution: 1:200.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse duodenum. Citrate synthetase antibody [1761] dilution: 1:200.
Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648]

Immunohistochemistry-Paraffin: CS Citrate Synthase Antibody (1761) [NBP2-43648] - Analysis of mouse heart. Citrate synthetase antibody [1761] dilution: 1:200.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

Whole Japanese medaka extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

Citrate synthetase antibody [GT1761] detects CS protein by western blot analysis.
A. 30 ug zebrafish heart lysate/extract
10% SDS-PAGE
Citrate synthetase antibody [GT1761] (NBP2-43648) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Western Blot: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: CS Citrate Synthase Antibody (1761) [NBP2-43648] -

Citrate synthetase antibody [GT1761] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase protein stained by Citrate synthetase antibody [GT1761] (NBP2-43648) diluted at 1:200.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.

Applications for CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP2-43648 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CS Citrate Synthase

The protein encoded by the CS Citrate Synthase gene is a Krebs tricarboxylic acid cycle enzyme that catalyzes the synthesis of citratefrom oxaloacetate and acetyl coenzyme A. The enzyme is found in nearly all cells capable of oxidative metablism. Thisprotein is nuclear encoded and transported into the mitochondrial matrix, where the mature form is found. (provided byRefSeq)

Long Name

CS Citrate Synthase

Alternate Names

citrate (Si)-synthase, citrate synthase, mitochondrial, EC 2.3.3.1

Gene Symbol

CS

Additional CS Citrate Synthase Products

Product Documents for CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CS Citrate Synthase Antibody (1761) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CS Citrate Synthase Antibody (1761) - Azide and BSA Free

Customer Reviews for CS Citrate Synthase Antibody (1761) - Azide and BSA Free (1)

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  • CS Citrate Synthase Antibody (1761)
    Name: Hayk Simonyan
    Application: Western Blot
    Sample Tested: mouse liver lysate
    Species: Mouse
    Verified Customer | Posted 05/12/2017
    Primary antibody dilution 1:500. Image of mouse liver lysate.
    CS Citrate Synthase Antibody (1761) - Azide and BSA Free NBP2-43648

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