CTCF Antibody (4W9B0)

Novus Biologicals | Catalog # NBP3-15795

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation Sequencing

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4W9B0 expressed in HEK293
View all CTCF Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711). PAVEIEPEPEPQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQPAATDAPNGDLTPEMILSMMDR

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CTCF Antibody (4W9B0)

Western Blot: CTCF Antibody (4W9B0) [NBP3-15795]

Western Blot: CTCF Antibody (4W9B0) [NBP3-15795]

Western Blot: CTCF Antibody (4W9B0) [NBP3-15795] - Western blot analysis of extracts of various cell lines, using CTCF antibody (NBP3-15795) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795]

Immunocytochemistry/ Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795]

Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of NIH-3T3 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Chromatin immunoprecipitation analysis of extracts of 293T cells, using CTCF antibody (NBP3-15795) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Immunocytochemistry/ Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795]

Immunocytochemistry/ Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795]

Immunocytochemistry/Immunofluorescence: CTCF Antibody (4W9B0) [NBP3-15795] - Immunofluorescence analysis of C6 cells using CTCF antibody (NBP3-15795). Blue: DAPI for nuclear staining.
Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795]

Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795]

Immunoprecipitation: CTCF Antibody (4W9B0) [NBP3-15795] - Immunoprecipitation analysis of 300ug extracts of 293T cells using 3ug CTCF antibody (NBP3-15795). Western blot was performed from the immunoprecipitate using CTCF antibody (NBP3-15795) at a dilition of 1:1000.

Applications for CTCF Antibody (4W9B0)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

3μg antibody for 10 ug-15 ug of chromatin

Chromatin Immunoprecipitation Sequencing

1:50 - 1:100

ELISA

Recommended starting concentration is 1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:200 - 1:800

Immunoprecipitation

0.5μg-4μg antibody for 200 ug-400 ug extracts of whole cells

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CTCF

The CTCF gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA tar

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Gene Symbol

CTCF

Additional CTCF Products

Product Documents for CTCF Antibody (4W9B0)

Certificate of Analysis

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Product Specific Notices for CTCF Antibody (4W9B0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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