CTCF Antibody (CL0304) - BSA Free

Novus Biologicals | Catalog # NBP2-52909

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0304

Format

BSA Free
View all CTCF Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P49711, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for CTCF Antibody (CL0304) - BSA Free

Western Blot: CTCF Antibody (CL0304) [NBP2-52909]

Western Blot: CTCF Antibody (CL0304) [NBP2-52909]

Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-CTCF antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909]

Immunocytochemistry/ Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909]

Immunocytochemistry/Immunofluorescence: CTCF Antibody (CL0304) [NBP2-52909] - Staining of MCF7 cells using the Anti-CTCF monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human testis shows moderate nuclear positivity in a subset of cells in seminiferous ducts.
Western Blot: CTCF Antibody (CL0304) [NBP2-52909]

Western Blot: CTCF Antibody (CL0304) [NBP2-52909]

Western Blot: CTCF Antibody (CL0304) [NBP2-52909] - Lane 1: Marker [kDa], Lane 2: Human cell line U-251 MG
Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human endometrium shows moderate to strong nuclear positivity in glandular and stromal cells.
Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and glomeruli.
Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909]

Immunohistochemistry-Paraffin: CTCF Antibody (CL0304) [NBP2-52909] - Staining of human small intestine shows moderate nuclear positivity in glandular cells.
CTCF Antibody (CL0304) - BSA Free Chromatin Immunoprecipitation-exo-Seq: CTCF Antibody (CL0304) - BSA Free [NBP2-52909]

Chromatin Immunoprecipitation-exo-Seq: CTCF Antibody (CL0304) - BSA Free [NBP2-52909]

ChIP-Exo-Seq composite graph for Anti-CTCF (NBP2-52909) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for CTCF Antibody (CL0304) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTCF

The CTCF gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA tar

Long Name

CCCTC-binding Factor

Alternate Names

11-Zinc Finger Protein2, CCCTC-binding Factor

Gene Symbol

CTCF

Additional CTCF Products

Product Documents for CTCF Antibody (CL0304) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CTCF Antibody (CL0304) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

No product specific FAQs exist for this product.

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