CUGBP1/CELF1 Antibody (3B1) - BSA Free

Novus Biologicals | Catalog # NB200-316

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Primate, Rabbit

Cited:

Human, Mouse, Rat, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western, Immunoprecipitation, Gel Super Shift Assays

Cited:

Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, EMSA, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 3B1

Format

BSA Free
View all CUGBP1/CELF1 Primary Antibodies »

Product Specifications

Immunogen

Full-length human CUGBP1 [UniProt# Q92879]

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CUGBP1/CELF1 Antibody (3B1) - BSA Free

Western Blot: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Western Blot: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Western Blot: CUGBP1 Antibody (3B1) [NB200-316] - Detection of CUG-BP1 in several cell lysates.
Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunocytochemistry/ Immunofluorescence: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunocytochemistry/Immunofluorescence: CUGBP1 Antibody (3B1) [NB200-316] - Detection of the subcellular distribution of CUGBP1 (nuclear, non-nucleolar) in normal and DM1 (dystrophia myotonica) myoblasts.
Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Immunohistochemistry-Paraffin: CUGBP1/CELF1 Antibody (3B1) [NB200-316] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human spleen using 1:100 conc. of CUGBP1/CELF1 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Nuclear staining was observed in lymphocytes.
Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Flow Cytometry: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Flow Cytometry: CUGBP1 Antibody (3B1) [NB200-316] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using CUGBP1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
Simple Western: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Simple Western: CUGBP1/CELF1 Antibody (3B1) [NB200-316]

Simple Western: CUGBP1 Antibody (3B1) [NB200-316] - Simple Western lane view shows a specific band for CUGBP1 in 0.5 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for CUGBP1/CELF1 Antibody (3B1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1 ug per million cells

Gel Super Shift Assays

reported in scientific literature

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:100-1:500

Immunohistochemistry-Frozen

1:100-1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

reported in scientific literature

Simple Western

1:200

Western Blot

1:500
Application Notes
In Western Blot, a band is observed at approx. 50 kDa. Nuclear staining can be seen in ICC/IF.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:200, apparent MW was 55 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CUGBP1/CELF1

CUGBP1 (CUG-binding protein 1) is a member of CELF (CUG-BP- and ETR-3-like factors) family of RNA-binding proteins that binds to a variety of mRNA cis-elements including UG-rich elements, GC-rich elements, GU-rich elements and AREs, and has been implicated in the regulation of mRNA alternative splicing as well as the control of mRNA translation and stability. CUGBP1 is ubiquitously expressed with localization in nucleus and cytoplasm as a component of an EIF2 complex which contains CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. CUGBP1 also associates with polysomes and interacts with HNRNPH1 and PARN. CUGBP1 was originally discovered as a protein which interacts with RNA CUG repeats that are expanded in patients with DM1 (myotonic dystrophy), which binds to 3'-untranslated region of the dystrophin myotonia protein kinase (DMPK) gene. However, CUGBP1 was later shown as multifunctional protein regulating many posttranscriptional processes including alternative splicing, deadenylation, mRNA decay, and translation. It has been involved in the processes of development (embryonic and cardiac), differentiation (skeletal muscle and adipose tissue), germ cell formation, tumorigenesis (breast cancer, leukemia etc) and deterioration of certain tumors as well.

Long Name

CUGBP, Elav-Like Family Member 1

Alternate Names

BRUNOL2, CELF1, CUG-BP, CUG-BP1, D2Wsu101e, EDEN-BP, HNAB50, NAB50, NAPOR

Entrez Gene IDs

10658 (Human); 13046 (Mouse); 362160 (Rat)

Gene Symbol

CELF1

UniProt

Q92879

Additional CUGBP1/CELF1 Products

Product Documents for CUGBP1/CELF1 Antibody (3B1) - BSA Free

Certificate of Analysis

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Product Specific Notices for CUGBP1/CELF1 Antibody (3B1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Autophagy

Citations for CUGBP1/CELF1 Antibody (3B1) - BSA Free

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Protocols

View specific protocols for CUGBP1/CELF1 Antibody (3B1) - BSA Free (NB200-316):


Procedure Guide for NB 200-316 Monoclonal Anti-CUG

Western Blot Procedure

1) Wet Nitrocellulose membrane with PBS + NP40 [PN].
2) Pour off PN.
3) Dilute anti-CUG (catalog# NB 200-316) in 5%NFDM + NP40.
4) Incubate the primary antibody with the membrane for 1 hour, at room temperature (RT), gently rocking.
5) Wash 1x with PN for 10 minutes. Wash 2x with PN for 5 minutes, each.
6) Dilute anti-mouse-HRP (Amersham) in 5%NFDM + NP40 at 1:5,000.
7) Incubate the secondary antibody with the membrane for 45 minutes, at RT, gently rocking.
8) Wash 1x with PN for 10 minutes. Wash 2x with PN for 5 minutes, each.
9) Wash 1x with PBS for 5 minutes.
10) Develop using Amersham ECL components.
NOTE: HeLa nuclear cell extracts can be used as a positive control for this antibody.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for CUGBP1/CELF1 Antibody (3B1) - BSA Free

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  • Q: We have a question about one of your products: Has the epitope been mapped for this antibody (NB 200-316)? Thanks for any additional information you may be able to provide.

    A: We have not epitope mapped this antibody, so no further information is available.

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