Cullin 5 Antibody - N-terminal

Novus Biologicals | Catalog # NBP1-78041

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum
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Product Specifications

Immunogen

This Cullin 5 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an N-Terminal region near amino acids 1-25 of Human Cullin 5 (N-terminus) coupled to KLH. (Uniprot: Q93034)

Reactivity Notes

This product reacts with human Cullin 5. Cross reactivity is expected against mouse Cul5 based on a high degree of sequence homology. Cross reactivity with other human cullins may occur.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cullin 5 Antibody - N-terminal

Immunohistochemistry: Cullin 5 Antibody - N-terminal [NBP1-78041]

Immunohistochemistry: Cullin 5 Antibody - N-terminal [NBP1-78041]

Immunohistochemistry: Cullin 5 Antibody [NBP1-78041] - Cullin 5 antibody was diluted 1:500 to detect Cullin 5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
N/A: Cullin 5 Antibody [NBP1-78041]
Cullin 5 Antibody - N-terminal

Cullin 5 Antibody - N-terminal

Anti-CUL5 antibody was diluted 1:500 to detect CUL5 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Applications for Cullin 5 Antibody - N-terminal

Application
Recommended Usage

ELISA

1:2000-1:10000

Immunohistochemistry

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:500-1:1000
Application Notes
This product has been tested by immunohistochemistry. This antibody reacts with human Cul5 by western blot and immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using 293T, U2OS and others). This antibody also co-immunoprecipitates associated proteins. A 90.9 kDa band corresponding to human Cul5 is detected. Most cell lines expressing Cul4A can be used as a positive control. Researchers should determine optimal titers for other applications.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Cullin 5

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.

Alternate Names

CUL-5, cullin 5, VACM-1cullin-5, VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1), Vasopressin-activated calcium-mobilizing receptor 1, Vasopressin-activated calcium-mobilizing receptor-1

Gene Symbol

CUL5

UniProt

Q93034 (Human)

Additional Cullin 5 Products

Product Documents for Cullin 5 Antibody - N-terminal

Certificate of Analysis

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Product Specific Notices for Cullin 5 Antibody - N-terminal

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Cullin 5 Antibody - N-terminal

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  • Q: Could you let us know whether it's supplied in the form of whole serum?

    A: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. In other word that no further purification has been done after the lipid and fibrinogen components were moved from this antiserum, followed by the filter sterilization. Therefore the answer is yes, it should be a whole serum-type of the antibody product.

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