CXCL4/PF4 Antibody (6Q10H2)

Novus Biologicals | Catalog # NBP3-16251

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6Q10H2 expressed in HEK293
View all CXCL4/PF4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-101 of human CXCL4/CXCL4/PF4 (P02776). MSSAAGFCASRPGLLFLGLLLLPLVVAFASAEAEEDGDLQCLCVKTTSQVRPRHITSLEVIKAGPHCPTAQLIATLKNGRKICLDLQAPLYKKIIKKLLES

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

11 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCL4/PF4 Antibody (6Q10H2)

Western Blot: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Western Blot: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Western Blot: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Western blot analysis of extracts of various cell lines, using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Immunohistochemistry of paraffin-embedded rat lung using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251]

Immunohistochemistry-Paraffin: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Immunohistochemistry of paraffin-embedded mouse bone marrow using CXCL4/CXCL4/PF4 Rabbit mAb (NBP3-16251) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
CXCL4/PF4 Antibody (6Q10H2)

Immunocytochemistry/ Immunofluorescence: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] -

Immunocytochemistry/ Immunofluorescence: CXCL4/PF4 Antibody (6Q10H2) [NBP3-16251] - Confocal imaging of Mouse leg bone using CXCL4/PF4 Rabbit mAb. DAPI was used for nuclear staining (blue). Objective: 40x.

Applications for CXCL4/PF4 Antibody (6Q10H2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:100 - 1:1000

Immunohistochemistry-Paraffin

1:100 - 1:1000

Western Blot

1:1000 - 1:4000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CXCL4/PF4

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair (Eisman et al., 1990 [PubMed 1695112]).[supplied by OMIM]

Alternate Names

PF4

Gene Symbol

PF4

Additional CXCL4/PF4 Products

Product Documents for CXCL4/PF4 Antibody (6Q10H2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CXCL4/PF4 Antibody (6Q10H2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CXCL4/PF4 Antibody (6Q10H2)

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Protocols

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FAQs

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