CXCR7/RDC-1 Antibody

Novus Biologicals | Catalog # NBP1-31309

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Predicted:

Canine (94%), Rhesus Macaque (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, FC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human CXCR7/RDC-1. The exact sequence is proprietary.

Reactivity Notes

Bovine (81%).

Localization

Cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309]

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309]

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunocytochemistry/ Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunocytochemistry/Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309] - Analysis of human pancreatic cancer cell line, using CCR7 antibody at 1:50 dilution. Blue: DAPI.
Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry-Paraffin: CXCR7/RDC-1 Antibody [NBP1-31309] - Human gastric cancer, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309]

Immunohistochemistry: CXCR7/RDC-1 Antibody [NBP1-31309] - Human pancreatic tumor, using CXCR7 antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] - Unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301)) was used to detect the primary antibody.
CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
CXCR7/RDC-1 Antibody

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -

Western Blot: CXCR7/RDC-1 Antibody [NBP1-31309] -Boiled and unboiled Raji whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [C1C2], Internal diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)
CXCR7/RDC-1 Antibody

Immunocytochemistry/ Immunofluorescence: CXCR7/RDC-1 Antibody [NBP1-31309] -

CXCR7/RDC-1 antibody [C1C2], Internal detects CXCR7/RDC-1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7/RDC-1 stained by CXCR7/RDC-1 antibody [C1C2], Internal (NBP1-31309) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for CXCR7/RDC-1 Antibody

Application
Recommended Usage

ELISA

Assay dependent

Flow Cytometry

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

Assay dependent

Western Blot

1:500-1:3000

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CXCR7/RDC-1

CCR7, or C-C chemokine receptor type 7, is a G protein-coupled, seven-transmembrane domain receptor protein. Originally identified as the EBV-induced gene-1 (1), CCR7 was later found to be the receptor for the chemokines CCL21 (SLC, 6Ckine, Exodus-2) (3) and CCL19 (MIP-3

Alternate Names

ACKR3, CMKOR1, CXCR7, GPR159, RDC-1

Entrez Gene IDs

1236 (Human)

Gene Symbol

CCR7

UniProt

Additional CXCR7/RDC-1 Products

Product Documents for CXCR7/RDC-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CXCR7/RDC-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for CXCR7/RDC-1 Antibody

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Protocols

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