Cyclin A2 Antibody

Novus Biologicals | Catalog # NB600-495

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum
View all Cyclin A2 Primary Antibodies »

Product Specifications

Immunogen

Cyclin A2 Antibody was produced by repeated immunizations with a recombinant protein corresponding to the human cyclin A. (Uniprot: P20248)

Reactivity Notes

Antiserum will specifically react with a 60 kDa Cyclin A2 protein from human, rat and mouse tissue. No reaction was observed against other related cyclins. Cross reactivity with Cyclin A2 from other species may also occur.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This antibody was prepared from monospecific antiserum by delipidation and defibrination

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Cyclin A2 Antibody

Western Blot: Cyclin A2 Antibody [NB600-495]

Western Blot: Cyclin A2 Antibody [NB600-495]

Western Blot: Cyclin A2 Antibody [NB600-495] - Lane 1: T98G cells treated with normal (n). Lane 2: T98G cells treated with scrambled (scr). Lane 3: T98G cells treated with PIN1 knockdown (kd1). Load: 25 ug per lane. Primary antibody: Cyclin A antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 60kDa for Cyclin A.
Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495]

Immunohistochemistry-Paraffin: Cyclin A2 Antibody [NB600-495] - Anti-Cyclin A antibody was diluted 1:500 to detect Cyclin A in human testes tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Applications for Cyclin A2 Antibody

Application
Recommended Usage

ELISA

1:5000-1:20000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:100

Western Blot

1:500-1:5000
Application Notes
This product has been tested by western blot and immunohistochemistry and is suitable for the detection by immunoblot, ELISA, IHC, and IP of human, rat and mouse Cyclin A. Researchers should determine optimal titers for applications that are not stated below.

Formulation, Preparation, and Storage

Purification

Delipidation and Defibrination

Formulation

Antiserum

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Cyclin A2

Cyclins are regulatory subunits of the cyclin-dependent kinases (cdks) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdks. These cyclin / cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S phase and passage through G2 phase.

Alternate Names

CCNA, CCNA2

Entrez Gene IDs

890 (Human); 12428 (Mouse); 114494 (Rat)

Gene Symbol

CCNA2

UniProt

P20248 (Human)

Additional Cyclin A2 Products

Product Documents for Cyclin A2 Antibody

Certificate of Analysis

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Product Specific Notices for Cyclin A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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