Cyclophilin B Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85358

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cyclophilin B Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cyclophilin B Antibody - BSA Free

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining in human thyroid gland and skeletal muscle tissues using anti-PPIB antibody. Corresponding PPIB RNA-seq data are presented for the same tissues.
Western Blot: Cyclophilin B Antibody [NBP1-85358]

Western Blot: Cyclophilin B Antibody [NBP1-85358]

Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Western Blot: Cyclophilin B Antibody [NBP1-85358]

Western Blot: Cyclophilin B Antibody [NBP1-85358]

Western Blot: Cyclophilin B Antibody [NBP1-85358] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Cyclophilin B Antibody [NBP1-85358]

Immunocytochemistry/ Immunofluorescence: Cyclophilin B Antibody [NBP1-85358]

Immunocytochemistry/Immunofluorescence: Cyclophilin B Antibody [NBP1-85358] - Staining of human cell line HUVEC TERT2 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human placenta shows strong granular cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human thyroid gland shows high expression.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human prostate shows weak granular cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358]

Immunohistochemistry-Paraffin: Cyclophilin B Antibody [NBP1-85358] - Staining of human endometrium shows moderate granular cytoplasmic positivity in glandular cells.

Applications for Cyclophilin B Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Simple Western

8 ug/mL

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
See Simple Western Antibody Database for Simple Western validation: Tested in Titrated to saturation using various models, separated by Size, antibody dilution of 8 ug/mL

Reviewed Applications

Read 1 review rated 4 using NBP1-85358 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cyclophilin B

Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, Cyclophilin B mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human Cyclophilin B precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27-34), a PPIase domain (aa 47-204) and a C-terminal ER retention motif (aa 213-216). Over aa 34-216, the human and mouse sequences are 95% aa identical.

Alternate Names

CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP

Gene Symbol

PPIB

Additional Cyclophilin B Products

Product Documents for Cyclophilin B Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cyclophilin B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cyclophilin B Antibody - BSA Free

Customer Reviews for Cyclophilin B Antibody - BSA Free (1)

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  • Cyclophilin B Antibody
    Name: Rob Wilpan
    Application: Simple Western
    Sample Tested: mouse liver lysate
    Species: Mouse
    Verified Customer | Posted 04/21/2017
    Used NBP1-85358 anti-Cyclophilin B antibody with B6 Mouse Liver Lysate @ 1mg/ml because of the lack of sensitivity of another antibody. Runs as 28 kDa on the 12 to 230 kDa kit. Antibody titration of 1:6.25 to 1:200 is shown.
    Cyclophilin B Antibody - BSA Free NBP1-85358

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