CYP51A1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-48733

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Rat (92%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CYP51A1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: RSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFYKAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQH

Reactivity Notes

Mouse (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CYP51A1 Antibody - BSA Free

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex, colon, liver and testis using Anti-CYP51A1 antibody NBP2-48733 (A) shows similar protein distribution across tissues to independent antibody NBP2-48793 (B).
Western Blot: CYP51A1 Antibody [NBP2-48733]

Western Blot: CYP51A1 Antibody [NBP2-48733]

Western Blot: CYP51A1 Antibody [NBP2-48733] - Analysis in Caco-2 cells transfected with control siRNA, target specific siRNA probe #1 and #2. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: CYP51A1 Antibody [NBP2-48733]

Immunocytochemistry/ Immunofluorescence: CYP51A1 Antibody [NBP2-48733]

Immunocytochemistry/Immunofluorescence: CYP51A1 Antibody [NBP2-48733] - Staining of human cell line A-431 shows localization to endoplasmic reticulum. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis using Anti-CYP51A1 antibody NBP2-48733.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human skeletal muscle shows no cytoplasmic positivity in myocytes.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human cerebral cortex.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human liver.
Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733]

Immunohistochemistry-Paraffin: CYP51A1 Antibody [NBP2-48733] - Staining of human colon.

Applications for CYP51A1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CYP51A1

CYP51A1, also known as Lanosterol 14-alpha demethylase, consists of a 56.8 kDa and a 46.3 kDa isoform and is involved in transforming lanosterol by removing the 14alpha-methyl group. Current research is being conducted with several diseases including tuberculosis, antley-bixler syndrome, colorectal cancer, leukemia, immunodeficiency, prostatitis, cholesterol, and chagas disease. This protein has been linked to the cholesterol and steroid biosynthesis, metabolism, and signaling pathways, where it interacts with FA2H, MSMO1, SCD, ZMPSTE24, and FDFT1.

Long Name

Lanosterol 14-alpha demethylase

Alternate Names

CYP51, CYPLI, Cytochrome P45014DM, Cytochrome P450LI, EC 1.14.14.154, LDM

Gene Symbol

CYP51A1

Additional CYP51A1 Products

Product Documents for CYP51A1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CYP51A1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CYP51A1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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