Cystathionase Antibody (S51) - Azide and BSA Free

Novus Biologicals | Catalog # H00001491-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # S51

Format

Azide and BSA Free
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Product Specifications

Immunogen

CTH (AAH15807, 1 a.a. ~ 405 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEKDASSQGFLPHFQHFATQAIHVGQDPEQWTSRAVVPPISLSTTFKQGAPGQHSGFEYSRSGNPTRNCLEKAVAALDGAKYCLAFASGLAATVTITHLLKAGDQIICMDDVYGGTNRYFRQVASEFGLKISFVDCSKIKLLEAAITPETKLVWIETPTNPTQKVIDIEGCAHIVHKHGDIILVVDNTFMSPYFQRPLALGADISMYSATKYMNGRSDVVMGLVSVNCESLHNRLRFLQNSLGAVPSPIDCYLCNRGLKTLHVRMEKHFKNGMAVAQFLESNPWVEKVIYPGLPSHPQHELVKRQCTGCTGMVTFYIKGTLQHAEIFLKNLKLFTLAESLGGFESLAELPAIMTHASVLKNDRDVLGISDTLIRLSVGLEDEEDLLEDLDQALKAAHPPSGSHS

Reactivity Notes

Rat reactivity reported in customer review. Rat reactivity reported in scientific literature (PMID: 22801977). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Localization

Cytoplasmic

Specificity

CTH - cystathionase (cystathionine gamma-lyase)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Cystathionase Antibody (S51) - Azide and BSA Free

Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in transfected 293T cell line by CTH monoclonal antibody (M03), clone S51. Lane 1: CTH transfected lysatE (45 KDa). Lane 2: Non-transfected lysate.
Immunohistochemistry-Paraffin: Cystathionase Antibody (S51) [H00001491-M03]

Immunohistochemistry-Paraffin: Cystathionase Antibody (S51) [H00001491-M03]

Immunohistochemistry-Paraffin: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of monoclonal antibody to CTH on formalin-fixed paraffin-embedded human pancreas. Antibody concentration 3 ug/ml
Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in K-562 (Cat # L009V1).
Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03]

Western Blot: Cystathionase Antibody (S51) [H00001491-M03] - Analysis of CTH expression in mouse kidney.
ELISA: Cystathionase Antibody (S51) [H00001491-M03]

ELISA: Cystathionase Antibody (S51) [H00001491-M03]

ELISA: Cystathionase Antibody (S51) [H00001491-M03] - Detection limit for recombinant GST tagged CTH is approximately 1ng/ml as a capture antibody.

Applications for Cystathionase Antibody (S51) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate, transfected lysate and recombinant protein for WB. It has been use for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cystathionase

This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.

Alternate Names

cystathionase (cystathionine gamma-lyase), cystathionine gamma-lyase, cysteine desulfhydrase, EC 4.4.1, EC 4.4.1.1, gamma-cystathionase, homoserine deaminase, homoserine dehydratase, MGC9471

Entrez Gene IDs

1491 (Human)

Gene Symbol

CTH

OMIM

219500 (Human)

Additional Cystathionase Products

Product Documents for Cystathionase Antibody (S51) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Cystathionase Antibody (S51) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cystathionase Antibody (S51) - Azide and BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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